TruSight Cardio Sequencing Panel

此试剂盒为研究人员提供了鉴定遗传性心脏病(ICC)致病变异的全面、经济的解决方案。 阅读更多...
选择产品
Enrichment Panels

TruSight Cardio – Enrichment Oligos only (8 Enrichment Reactions)

20029229


Library Prep

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

配件产品

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

IDT for Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 indexes, 96 samples)

20027214

IDT for Nextera DNA Unique Dual Indexes Set C, Tagmentation (96 indexes, 96 samples)

20027215

IDT for Nextera DNA Unique Dual Indexes Set D, Tagmentation (96 indexes, 96 samples)

20027216

产品特色

  • Expertly defined genes selected in collaboration with the Imperial College of London
  • 99% of the targeted regions covered at a depth of at least 20×*
  • Cost to sequence is ~ $1 US per gene; fully supported on Illumina sequencing and informatics platforms

The TruSight Cardio Kit uses next-generation sequencing (NGS) to provide comprehensive coverage of 174 genes with known associations to 17 ICCs, including cardiomyopathies, arrhythmias, aortopathies, and more. Genes were expertly selected with researchers at the National Heart Centre Singapore and Imperial College of London. Content includes genes known to be associated with hereditary heart disease and emerging genes found in the literature.1

经常一并购买

Plan on Using the TruSight Cardio Sequencing Kit with MiSeq v3?

Download this reference guide to understand which kit quantities you should purchase to support your research.

Download Reference Guide
Reference guide: TruSight Cardio Sequencing Kit

规格

计划推荐

仪器 推荐的样本数 读长
MiSeq System 12 samples per run with v2 reagents (based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)
NextSeq 550 System 48 samples per run (mid output; based on 300x mean coverage of targeted content) 2 × 150 bp (max recommended)

产品比较

TruSight Cardio Sequencing Panel TruSight One Sequencing Panels
内容说明 575 Kb基因组内容(174个基因) TruSight One:约12 Mb基因组内容(约4800个基因)。
TruSight One Expanded:约16.5 Mb基因组内容(约6700个基因)。
多重分析 多达96重 多达96重
方法 靶向富集, 靶向DNA测序 靶向富集, 靶向DNA测序
物种类别 人类 人类
特殊的样本类型 Not FFPE-Compatible Not FFPE-Compatible
说明 此款靶向测序研究panel有助于鉴定与17种遗传性心脏病相关的遗传变异。 固定基因集的panel,专注于具有致病变异的外显子区域。
起始量 50 ng DNA 50 ng DNA

Method-Specific Workflow Example

 

The TruSight Cardiomyopathy Sequencing Panel has been discontinued. The TruSight Cardio Sequencing Kit is the recommended replacement.

Related Products to Consider

TruSight One Sequencing Panels

此Panel专注于含致病变异的外显子区域。其可让实验室通过一个实验分析方法代替多种不同的分子实验分析方法。


Illumina DNA Prep with Enrichment

广泛适用于各种应用的快速、集成的工作流程,能为定制panel、固定基因集的panel和全外显子组富集提供靶向重测序。


MiSeq Reagent Kits v2

MiSeq预装即用型测序试剂卡盒。对于低产出应用,提供micro和nano两种规格。


References
  1. Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Trans Res. 2016;9:3.

* Data on file. Illumina, Inc. 2015.