Nextera DNA Flex Library Prep Kit

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.Read More...
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What products do I need?
Library Prep

Nextera DNA Flex Library Prep (24 Samples)



Nextera DNA Flex Library Prep (96 Samples)



Index Adapters

Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)



Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)


Accessory Products
What accessories do I need?

Flex Lysis Reagent Kit



Illumina® Free Adapter Blocking Reagent (12 reactions)



Illumina® Free Adapter Blocking Reagent (48 reactions)



Product Highlights

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

  • Fastest Illumina library prep workflow, with ~3.5 hours total time
  • Flexibility to accommodate variations in sample type, DNA input amount, and application
  • Optimized library prep performance, generating reliable results
Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.

*Demonstrated protocols available.

Explore the Next Era in Library Prep

Discover the power Nextera DNA Flex can bring to your lab through this interactive experience. This library prep kit offers whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic
Introduction to Nextera DNA Flex Library Prep Kit


Method-Specific Workflow Example


Supporting Data and Figures

How It Works

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