NextSeq 500 System

Current Consumables

NextSeq 500/550 High Output Kit—Available in 3 sizes (300 cycles, 150 cycles, and 75 cycles), the NextSeq 500/550 High Output Kit includes a high-output flow cell, a high-output reagent cartridge, and a buffer cartridge.

NextSeq 500/550 Mid Output Kit—Available in 2 sizes (300 cycles and 150 cycles), the NextSeq 500/550 Mid Output Kit includes a mid-output flow cell, a mid-output reagent cartridge, and a buffer cartridge.

Kit Versions and Compatibility—The NextSeq 500/550 Kit v2 requires NCS v1.4, or later. If using the original NextSeq 500 Kit (v1), continue using NCS v1.3.

Current Software

NextSeq Control Software—The control software interface guides you through the steps to load the flow cell and reagents before beginning a sequencing run. During the run, the control software operates the imaging stage, dispenses reagents, controls flow cell temperatures, and captures images of clusters on the flow cell.

Real-Time Analysis—The NextSeq system uses an updated implementation of Real-Time Analysis (RTA) called RTA v2, which includes important architecture differences from RTA used on other Illumina sequencing systems. Unlike earlier versions of RTA, all processes are performed in memory to maximize processing speed. RTA is part of the NextSeq system software.

Analysis Solutions

The NextSeq system is enabled to integrate with BaseSpace, providing an end to end data storage, sharing, and analysis system. The NextSeq seamlessly streams data to BaseSpace, where demultiplexing and conversion to industry-standard FASTQ file formats occurs automatically as the final step in data transfer to Basespace.

Use bcl2fastq2 Conversion Software to convert NextSeq sequencing data to standard FASTQ file formats for downstream analysis using third-party analysis solutions. For more information, see bcl2fastq2 Conversion Software.

Illumina NextSeq 500