HiSeq X Reagent Kits

Catalog IDs: FC-501-2501, FC-501-2521, FC-502-2501, FC-502-2521

Kit Requirements

Performing a sequencing run requires a single-use HiSeq X Ten Reagent Kit v2.5 or HiSeq X Five Reagent Kit v2.5. Each kit is available in two bundles:

  • Single-pack kit—Provides flow cells, reagents, and accessories for one dual flow cell run or two single flow cell runs.
  • Ten-pack kit—Provides flow cells, reagents, and accessories for 10 dual flow cell runs or 20 single flow cell runs.

When preparing consumables, check the coded labels:

  • PE cluster kit—Green rectangle marked PE
  • Flow cells—Green rectangle
  • SBS kit box—Blue dot

For other system requirements and compatible products, see HiSeq X Compatibility & Requirements.

Contents and Storage

Single-Pack Kit

The single-pack kit contains the following components. Store at the temperatures indicated in the following tables.

Quantity

Component

 

2 PE Cluster Kit v2 (Box 1 of 2)  
2 PE Cluster Kit v2 (Box 2 of 2)  
2 Patterned Flow Cell  
2 cBot Manifold  
2 SBS Kit v2 (Box 1 of 2)  
2 SBS Kit v2 (Box 2 of 2)  
2 Accessory Kit v2  
PE Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1 cBot Reagent Plate cBot Reagent Plate, PE
1 RSB Resuspension Buffer
1 EXP1 Enhanced Patterned Cluster Mix 1
1 EXP2 Enhanced Patterned Cluster Mix 2
1 EXP3 Enhanced Patterned Cluster Mix 3
PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1 PRM Oncology RNA Probes Master Pool
1 PLM v2 Patterned Linearization Mix 2
1 PAM Patterned Amplification Mix
1 PPM Patterned Amp Premix
1 PDR Patterned Denaturation Mix (contains formamide)
1 HP11 Read 2 Primer Mix
1 HP14 Index i5 and i7 Primer Mix
SBS Kit Box 1

Quantity

Reagent

Description

Storage

1 PW1 Wash Buffer -25°C to 30°C
1 PB1* Patterned SBS Buffer 1 15°C to 30°C
3 PB2 Patterned SBS Buffer 2 15°C to 30°C

* PB1 is sensitive to light. Store PB1 in a dark location.

SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1 PCM Patterned Cleavage Mix
1 PIM* Patterned Incorporation Mix
1 PSM Patterned Scan Mix

* PIM is sensitive to light. Store PIM in a dark location.

Accessory Kit, Store at Room Temperature

Quantity

Accessory

Purpose

1 Flow cell gaskets 
(package of 4)
Replace the flow cell gaskets between runs
1 Flow cell storage tube with storage buffer, 50 ml Store the flow cell between clustering and sequencing
1 Funnel bottle caps 
(package of 8)
Replace the caps on SBS reagent bottles before loading reagents
Ten-Pack Kit

The 10-pack kit contains the following components. Store at the temperatures indicated in the following tables.

Quantity

Component

 

1 PE Cluster Kit v2 (Box A)  
1 PE Cluster Kit v2 (Box B)  
1 PE Cluster Kit v2 (Box C)  
1 Patterned Flow Cell (Box of 20)  
20 cBot Manifold  
1 SBS Kit v2 (Box A)  
2 SBS Kit v2 (Box B)  
6 SBS Kit v2 (Box C)  
2 SBS Kit v2 (Box D)  
2 SBS Kit v2 (Box E)  
2 SBS Kit v2 (Box F)  
1 Accessory Kit v2 (Box A)  
1 Accessory Kit v2 (Box B)  
PE Cluster Kit Box A, Store at -25°C to -15°C

Quantity

Reagent

Description

20 cBot Reagent Plate cBot reagent plate, PE
PE Cluster Kit Box B, Store at -25°C to -15°C

Quantity

Reagent

Description

5 RSB Resuspension Buffer
5 EPX1M Enhanced Patterned Cluster Mix 1
5 EPX2M Enhanced Patterned Cluster Mix 2
5 EPX3M Enhanced Patterned Cluster Mix 3

Enhanced Patterned Cluster Mix 1

Enhanced Patterned Cluster Mix 1

PE Cluster Kit Box C, Store at -25°C to -15°C

Quantity

Reagent

Description

20 PRM Oncology RNA Probes Master Pool
20 PLM v2 Patterned Linearization Mix 2
20 PAM Patterned Amplification Mix
20 PPM Patterned Amp Premix
20 PDR Patterned Denaturation Mix (contains formamide)
20 HP11 Read 2 Primer Mix
20 HP14 Index i5 and i7 Primer Mix
SBS Kit Boxex A-F

Box

Reagent

Quantity

Description

Storage

SBS Box A PW1 20 each Wash Buffer -25°C to 30°C
SBS Box B PB1* 10 each Patterned SBS Buffer 1 15°C to 30°C
SBS Box C PB2 10 each Patterned SBS Buffer 2 15°C to 30°C
SBS Box D PCM 10 each Patterned Cleavage Mix -25°C to 30°C
SBS Box E PIM* 10 each Patterned Incorporation Mix -25°C to 30°C
SBS Box F PSM 10 each Patterned Scan Mix -25°C to 30°C

* PB1 and PIM are sensitive to light. Store in a dark location.

Accessory Kit Boxes A and B, Store at Room Temperature

Box

Accessory

Quantity

Purpose

Box A Flow cell storage tube with storage buffer, 50 ml 20 each Replace the flow cell gaskets between runs.
Box B Flow cell gaskets 
(package of 4)
20 each Store the flow cell between clustering and sequencing.
Box B Funnel bottle caps 
(package of 8)
20 each Replace the caps on SBS reagent bottles before loading reagents.