Kit Requirements & Contents

Contents & Storage

Kit Requirements

  • TruSeq Cluster Kit v3—Available in paired-end (PE) and single-read (SR) versions.
    • TruSeq Dual Index Sequencing Primer Box—Required in addition to TruSeq Cluster Kit v3 if sequencing Nextera libraries. Primers provided in the TruSeq Cluster Kit v3 are not compatible with Nextera libraries.
    • To perform dual-indexed sequencing on a single-read flow cell, HP9 is required. HP9 is provided in the PE version of the TruSeq Dual Index Sequencing Primer Box.
  • TruSeq SBS Kit v3

TruSeq Cluster Kit v3

PE Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HP3

Denaturation Solution

1

HT2

Wash Buffer

1

RMR

Resynthesis Mix

1

AT2

100% Formamide

1

APM2

Amplification Premix

1

AMX2

Amplification Mix 2

1

LMX2

Linearization Mix 2

1

BMX

Blocking Mix

1

HP7*

Read 2 Sequencing Primer Mix

* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.

PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Kit Component

Description

1

cBot Reagent Plate

cBot PE reagent plate

1

HT1

Hybridization Buffer

SR Cluster Kit Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Reagent Plate

cBot SR reagent plate

1

HT1

Hybridization Buffer

Multiplex Sequencing Primer Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HP3

Denaturation Solution

1

HT2

Wash Buffer

1

HP8*

Index Sequencing Primer

* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.

Accessory Kit

Quantity

Component

Purpose

4

Flow cell gaskets

Replace the flow cell gaskets between runs.

8

Funnel bottle caps

Replace SBS reagent bottle caps before loading reagents.

1

ICB instrument tube

Divide ICB for Read 2.

TruSeq SBS Kit v3

Reagents are labeled with a 3-letter acronym plus a numeric designator that indicates the kit size

 

200-Cycle SBS Kit Box 1

Quantity

Reagent

Description

Storage Temperature

1

PW1

Wash Buffer

-25°C to 30°C

1

ICB

Incorporation Mix Buffer

2°C to 8°C

1

SB1

High Salt Buffer

2°C to 8°C

2

SB2

Incorporation Wash Buffer

2°C to 8°C

1

SB3

Cleavage Buffer

2°C to 8°C

200-Cycle SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

CMR

Cleavage Mix Reagent

1

SRE

Enhanced Scan Mix Reagent

1

EDP

Enhanced DNA Polymerase

4

LFN36

Long Read Nucleotide Mix

50-Cycle SBS Kit Box 1

Quantity

Reagent

Description

Storage Temperature

1

PW1

Wash Buffer

-25°C to 30°C

1

ICB

Incorporation Mix Buffer

2°C to 8°C

1

SB1

High Salt Buffer

2°C to 8°C

2

SB2

Incorporation Buffer

2°C to 8°C

1

SB3

Cleavage Buffer

2°C to 8°C

50-Cycle SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

CMR

Cleavage Mix Reagent

1

SRE

Enhanced Scanning Mix Reagent

1

EDP

Enhanced DNA Polymerase

1

LFN36

Long Read Nucleotide Mix

Safety Data Sheets

See Safety Data Sheets.