TruSeq Cluster Kit v3 Support

Catalog IDs: PE-401-3001, GD-401-3001

Latest Updates

TruSeq Cluster Kit v3 Reagent Prep Guide


To cluster a flow cell on the cBot for subsequent sequencing on the HiSeq, you need 1 TruSeq Cluster Kit v3. Cluster Kits are available in paired-end (PE) and single-read (SR) versions.

The TruSeq Cluster Kit v3 is designed specifically for use with the TruSeq v3 high output mode on enabled systems.

If you are sequencing Nextera libraries, you need sequencing primers provided in the TruSeq Dual Index Sequencing Primer Box in addition to the TruSeq Cluster Kit v3. Primers provided in the TruSeq Cluster Kit v3 are not compatible with Nextera libraries.

To perform dual-indexed sequencing on a single-read flow cell, HP9 is required. HP9 is provided in the PE version of the TruSeq Dual Index Sequencing Primer Box.