TruSeq Nano DNA Library Prep Kit for NeoPrep Compatible Products

The NeoPrep System normalizes libraries to 10 nM. Use the following table to determine insert, concentration, and load size for your system and the resulting expected cluster density.

  • Dilute the normalized library to the predenature concentration. 
  • Denature and dilute the library to the intermediate concentration. Depending on the application, pooling after denaturation and dilution can increase library complexity due to the number of unique molecules present in the final mixture when each library is denatured separately in a larger volume.
  • Load the denatured samples using the pooled denatured samples load size. 
System Insert Size Predenatured Concentration (nM) Denatured/Diluted Intermediate Concentration (pM) Pooled Denatured Samples Load (pM) Expected Cluster Density (mm2)
MiSeq 350 4 40 10–14 600–1000
  550 4 40 12-16 600–1000
NextSeq 350 4 40 2-3 ~200
  550 4 40 2-3 ~200
HiSeq 2500 TruSeq v4 350 4 40 18-22 600-1000
  550 4 40 20-24 600-1000
HiSeq 2500 v3 350 4 40 18-22 600-1000
  550 4 40 20-24 600-1000
HiSeq 2500 Rapid Run (v1) 350 4 40 12-16 600-1000
  550 4 40 14-20 600-1000