Illumina DNA Prep

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.Read More...
Select Product(s)
What products do I need?
Library Prep

Illumina® DNA Prep, (M) Tagmentation (24 Samples)



Illumina® DNA Prep, (M) Tagmentation (96 Samples)



Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)



IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)



IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)



IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)



Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)



Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)




Illumina DNA Prep Training


Accessory Products
What accessories do I need?

Flex Lysis Reagent Kit (96 reactions)



Illumina® Free Adapter Blocking Reagent (12 Reactions)



Illumina® Free Adapter Blocking Reagent (48 Reactions)



NextSeq PhiX Control Kit



Product Highlights

Illumina DNA Prep offers flexibility for many whole-genome sequencing applications.

  • Fastest Illumina library prep workflow, with ~3.5 hours total time
  • Flexibility to accommodate variations in sample type, DNA input amount, and application
  • Optimized library prep performance, generating reliable results

Learn more about the technology

Save Time and Resources

Illumina DNA Prep uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Illumina DNA Prep workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*.

Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Illumina DNA Prep workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.

Access Flexible Throughput Options

The IDT for Illumina - DNA/RNA UD Indexes Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now. Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.

Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.

Related Notification

For whole-genome sequencing, Illumina DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.

*Demonstrated protocols available.

**Customer-reported application

Explore Illumina Library Prep

Discover the power that Illumina library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across Illumina sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic
Introduction to Nextera DNA Flex Library Prep Kit

Frequently Purchased Together


Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (high output; based on 30× coverage of a human genome) Up to 2 × 150 bp
NovaSeq 6000 System 2–10 samples per run  (dual flow cell; based on 30× coverage of a human genome) Up to 2 × 125 bp (rapid run) 
Up to 2 × 150 bp (high output)

Product Comparison

Method-Specific Workflow Example


Supporting Data and Figures

How It Works

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