Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement. Read More...
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Library Prep

Nextera XT DNA Library Preparation Kit (24 samples)

FC-131-1024

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Nextera XT DNA Library Preparation Kit (96 samples)

FC-131-1096

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Illumina Advantage Products

TG Nextera® XT DNA Sample Preparation Kit (96 Samples)

TG-131-1096

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TG Nextera® XT Index Kit v2 Set A (96 Indices, 384 Samples)

TG-131-2001

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TG Nextera® XT Index Kit v2 Set B (96 Indices, 384 Samples)

TG-131-2002

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Index Adapters

IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

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IDT® for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

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Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)

FC-131-2001

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Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)

FC-131-2002

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Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)

FC-131-2003

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Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)

FC-131-2004

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Nextera XT Index Kit (24 indexes, 96 samples)

FC-131-1001

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Accessory Products
What accessories do I need?

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

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Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

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Product Highlights

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
  • Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
  • Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
  • Recommended for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
  • Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single-tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It supports a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Learn more about the technology

Flexible Multiplexing

Multiplexing of up to 384 samples per Nextera XT library is available for projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

The IDT for Illumina-Nextera DNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing, which allows accurate assignment of reads and efficient use of the flow cell.  These unique dual index codes use 10 bp codes. This change in base pair index codes requires adjustments to the sequencing run setup.

Find robotic systems compatible with this kit

This product is also available as an Illumina Advantage (TG) product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

When to Use Standard vs. Bead-Based Normalization

For high-throughput research, bead-based normalization can save time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two normalization methods to help you decide which option to use.

Read Tech Note
When to Use Standard vs. Bead-Based Normalization

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiSeq System Up to 384 samples per run Up to 2 x 300 bp
NextSeq 550 System Up to 384 samples per run Up to 2 x 150 bp

Product Comparison

Nextera XT DNA Library Preparation Kit Illumina DNA Prep

Method-Specific Workflow Example

 

Customer Stories

Supporting Data and Figures

 

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