The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing studies.
Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.
Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.
Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.
View sample data (requires a BaseSpace login)
This kit has been validated for human and mouse samples. The BaseSpace Sequence Hub SureCell RNA Single-Cell App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.
Instrument | Recommended Number of Samples | Read Length |
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NextSeq 550 System | 4 to 6 samples per run (based on a minimum of 100,000 reads per cell) | read 1: 68 bp, read 2: 75 bp |
HiSeq 2500 System | Rapid run: 4-5 samples per lane; high output v4: 4 samples per lane (based on a minimum of 100,000 reads per cell) | read 1: 68 bp, read 2: 75 bp |
SureCell WTA 3′ Library Prep Kit for the ddSEQ System | TruSeq RNA Library Prep Kit v2 | Illumina Stranded mRNA Prep | Illumina RNA Prep with Enrichment |
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Single-Cell RNA Data Analysis Workflow
Technical Note | PDF< 1 MB
Illumina | Bio-Rad Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells
Application Note | PDF1 MB
Illumina Bio-Rad Single-Cell RNA Sequencing Solution
Data Sheet | PDF< 1 MB
SureCell WTA 3' Library Prep Kit Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions