Illumina DNA PCR-Free Prep is now available. This library prep kit achieves commensurate performance with TruSeq DNA PCR-Free, while reducing turnaround time and hands-on time.

TruSeq DNA PCR-Free

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.Read More...
Select Product(s)
What products do I need?
Library Prep

TruSeq DNA PCR-Free Low Throughput Library Prep Kit (24 samples)

20015962

Price
 
 

TruSeq DNA PCR-Free High Throughput Library Prep Kit (96 samples)

20015963

Price
 
 

Index Adapters

TruSeq DNA Single Indexes Set A (12 Indexes, 24 Samples)

20015960

Price
 
 

TruSeq DNA Single Indexes Set B (12 Indexes, 24 Samples)

20015961

Price
 
 

TruSeq DNA CD Indexes (96 Indexes, 96 Samples)

20015949

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples)

20020590

Price
 
 

IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples)

20022370

Price
 
 
Accessory Products

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Price
 
 

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Price
 
 

Product Highlights

TruSeq DNA PCR-Free provides simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole genomes. The workflows offer:

  • Shortened gel-free workflows that remove the need for PCR
  • Ability to sequence challenging regions
  • Excellent coverage quality for deep insight into the genome
Sequence challenging regions

TruSeq DNA PCR-Free offers superior coverage of areas that are traditionally difficult to sequence, such as GC-rich regions, promoters, and repetitive content.

The workflows are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Gain deep insight into the genome

PCR-free means reduced library bias and gaps. The result is high data quality and optimal variant detection across the genome. Excellent genome coverage quality means your results have few gaps and good coverage of GC-rich regions.

Save time with a PCR-free protocol

Removing PCR saves time and removes genomic coverage bias associated with PCR steps. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, TruSeq DNA PCR-Free provides a range of enhancements to a widely adopted library preparation workflow.

Access flexible throughput options
  • TruSeq DNA PCR-Free with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA PCR-Free with 96 CD Indexes supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.

Find a list of robotic systems that support the HT workflows

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (based on 30× coverage of a human genome)  350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp
HiSeq 2500 System 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome) 350 bp insert: ≤ 2 × 100 bp (rapid run)
550 bp insert: ≤ 2 × 250 bp (rapid run)
350 bp insert: ≤ 2 × 100 bp (high output)
550 bp insert: ≤ 2 × 125 bp (high output)
NovaSeq 6000 System Samples per run (dual flow cell): S1: 8, S2: 16, S4: 48 (based on 30× coverage of a human genome)  350 bp insert: ≤ 2 × 150 bp
550 bp insert: ≤ 2 × 150 bp

Product Comparison

Method-Specific Workflow Example

 

Customer Stories

Product Literature

Data Sheet | PDF | 5 versions

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

Related Products