TruSeq DNA Exome
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.Read More...
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TruSeq DNA Exome modular component options are discontinued. Alternative options are pre-assembled kits or Illumina DNA Prep with Enrichment with enhanced technology.
The TruSeq Exome Kit, previously known as the TruSeq Exome Library Prep Kit, is a cost-effective library preparation and exome enrichment solution that offers:
The TruSeq Exome Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and variant identification by sequencing up to 12 libraries per flow cell lane. This enables sequencing of more exomes per run, so researchers can maximize their budgets.
To learn more about calculating coverage estimates, see the sequencing coverage calculator.
The TruSeq Exome Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.
Illumina provides an integrated, supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.
Find automation vendors with robotic systems compatible with this product
Illumina is partnering with IDT to co-market a combined exome enrichment solution using the xGen® Exome Research Panel v1.0, which consists of 429,826 individually synthesized and quality-controlled xGen Lockdown® Probes. The panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled space. xGen Blocking Oligos (Cat. No.1075474, 1075475, or 1075476 for TruSeq libraries) and xGen Lockdown Reagents (Cat. No. 1072280 or 1072281) are also required.
Learn more about the IDT xGen Exome Research Panel
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | Samples per run: mid output: 3, high output: 12 (based on 50 million reads, 50× coverage, 4 Gb of data) | 2 × 75 bp (max recommended) |
| HiSeq 2500 System | Samples per run (dual flow cell): rapid run: 24, high output: 156 (based on 50 million reads, 50× coverage, 4 Gb of data) | 2 × 75 bp (max recommended) |
| NovaSeq 6000 System | Samples per run (dual flow cell): S1: 160, S2: 320, S4: 769 (based on 50 million reads, 50× coverage, 4 Gb of data) | ≤ 2 × 100 bp |
The TruSeq Exome Kit delivers ≥80% of on-target sequencing reads for efficient, cost-effective exome sequencing.
| Catalog Number | Total Samples | Number of Enrichment Reactions | Index Adapters |
|---|---|---|---|
| 20020614 | 24 | 8 | 24 single indexes (Set A and Set B) |
| 20020615 | 96 | 8 | 96 combinatorial dual indexes |
Evaluating DNA Quality from FFPE Samples
Technical Note | PDF< 1 MB
Illumina Exomes Comparative Table
Product Information Sheet | PDF< 1 MB
Data Sheet | PDF | 5 versions
Custom Protocol Selector
Generates customized, end-to-end instructions
Rapidly interrogate those portions of the human genome most important to your specific research.
This exome sequencing library preparation solution uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Targeted custom research panels optimized for sequencing specific targets or genomic content of interest.
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.
Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity.
