|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||5 to 16 samples per run (based on 25 million reads per sample)||2 x 75 bp|
|HiSeq 2500 System||24-160 samples per run (dual flow cell; based on 25 million reads per sample)||2 x 75 bp|
|NovaSeq 6000 System||Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations)||2 × 100 bp|
TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.