Limited-Time Offer: Save 25%

Achieve exceptionally rapid targeted RNA enrichment with the new Illumina RNA Prep with Enrichment. Use code RNA25 at checkout to receive 25% off.*

*Offer valid through June 30, 2021 for first purchase of a single library prep kit only. Index adapters and panels are not included in this promotion.

TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.Read More...
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Library Prep

TruSeq® RNA Library Prep for Enrichment (48 Samples)

20020189

Price
 
 

Enrichment

TruSeq® RNA Enrichment (12 enrichments)

20020490

Price
 
 

Index Adapters

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

Panel

Illumina Exome Panel – Enrichment Oligos Only

20020183

Price
 
 
Accessory Products

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Price
 
 

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Price
 
 

Product Highlights

TruSeq RNA Exome, previously known as the TruSeq RNA Access Library Prep Kit, converts total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissues and other low-quality samples.

  • Affordability and focus—Isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
  • High-quality data from difficult samples—Optimized for sequencing RNA from degraded samples, including FFPE tissues
  • Samples with limited starting material—Greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity

TruSeq RNA Exome generates RNA sequencing (RNA-Seq) libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions to help maximize discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis. Reagent volumes supplied are sufficient to support 1- to 4-plex enrichment reactions.

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Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 5 to 16 samples per run (based on 25 million reads per sample) 2 x 75 bp
HiSeq 2500 System 24-160 samples per run (dual flow cell; based on 25 million reads per sample) 2 x 75 bp
NovaSeq 6000 System Samples per run (dual flow cell): S1: 96, S2: 96, S4: 192 (currently limited by available index combinations) 2 × 100 bp

The product previously known as the TruSeq RNA Access Library Prep Kit (Cat. No. RS-301-2001 and RS-301-2002) is now called TruSeq RNA Exome. The product configuration has changed. In the new configuration, major components such as library preparation, index adapters, enrichment reagents, and exome panels can be purchased separately. If you were previously buying Cat. No. RS-301-2001 or Cat. No. RS-301-2002, purchase the following components: TruSeq RNA Library Prep for Enrichment (Cat. No. 20020189), TruSeq RNA Enrichment (Cat. No. 20020490), Exome Panel (Cat. No. 20020183), and one item from the Index Adapters section below.

Method-Specific Workflow Example

 

Customer Stories

Supporting Data and Figures

Efficient Gene Fusion Discovery
Supporting Data and Figures

TruSeq RNA Exome enables detection of expressed fusion transcripts without the need to design probes specific for the fusion junction. The well-characterized BCR-ABL fusion is detected efficiently in the Universal Human Reference RNA (UHRR) sample at 25 M reads.

 

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

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