TruSeq Stranded Total RNA with Ribo-Zero Plant

Automation capability	Liquid Handling Robots
Method	Whole-Transcriptome Sequencing
Variant class	Gene fusions, Transcript variants, Single nucleotide polymorphisms (SNPs), Novel transcripts
作用机制	基于磁珠的rRNA去除、cDNA合成和PCR
内容说明	捕获编码RNA以及多种形式的非编码RNA
多重分析	低通量试剂盒：混合最多12个样本。或混合24个样本（组A和B一起）, 高通量试剂盒：使用组合双标签序列或唯一双标签序列制备96个标签样本
技术	Illumina测序
核酸类型	RNA
物种	Plant
系统	NextSeq 550 Desktop Sequencer, HiSeq 3000, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 Desktop Sequencer, NovaSeq 6000 System, NovaSeq X Plus System, HiSeq 4000
起始量	0.1 – 1 ug从植物样本提取的高质量总RNA

Automation capability

Liquid Handling Robots

Method

Whole-Transcriptome Sequencing

Variant class

Gene fusions, Transcript variants, Single nucleotide polymorphisms (SNPs), Novel transcripts

作用机制

基于磁珠的rRNA去除、cDNA合成和PCR

内容说明

捕获编码RNA以及多种形式的非编码RNA

多重分析

低通量试剂盒：混合最多12个样本。或混合24个样本（组A和B一起）, 高通量试剂盒：使用组合双标签序列或唯一双标签序列制备96个标签样本

技术

Illumina测序

核酸类型

RNA

物种

Plant

系统

NextSeq 550 Desktop Sequencer, HiSeq 3000, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 Desktop Sequencer, NovaSeq 6000 System, NovaSeq X Plus System, HiSeq 4000

起始量

0.1 – 1 ug从植物样本提取的高质量总RNA

仪器	Recommended number of samples	Read length
NextSeq 550 System	RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)	2×75 bp 2×75 bp
NovaSeq 6000 System	RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.	≤ 2 × 100 bp ≤ 2 × 100 bp

仪器

Recommended number of samples

Read length

NextSeq 550 System

RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)

Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)

2×75 bp

2×75 bp

NovaSeq 6000 System

RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.

Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.

≤ 2 × 100 bp

≤ 2 × 100 bp

经常购买
其他需要考虑的产品

订购NovaSeq X系列

将先进的化学、光学和信息学技术相结合，实现了超凡的速度和数据质量、出色的通量和可扩展性。

MiSeq Reagent Kit v3

经过优化的试剂盒，可提供更高的簇密度和读长，与早期版本相比，提高了测序质量分值。

TruSight Oncology 500

靶向多种变异类型（包括微卫星不稳定性（MSI）和肿瘤突变负荷（TMB））的检测。