NextSeq 1000 & NextSeq 2000 Specifications

提高当前的测序效率,备以今后的规模需求

查看NextSeq 1000/1000-CN和NextSeq 2000/2000-CN测序系统的规格,从零开始重新设计,满足不断发展的需求

每个流动槽的测序产出*

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
流动槽类型 P1 P2 P3
1 × 50 bp     60 Gb
2 × 50 bp 10 Gb 40 Gb 120 Gb
2 × 100 bp   80 Gb 240 Gb
2 × 150 bp 30 Gb 120 Gb 360 Gb
2 × 300 bp 60 Gb 180 Gb
(300 M)
 

* 输出规格基于单个流动槽,使用Illumina PhiX对照文库,在支持的簇密度下产出进行计算。
† P3试剂仅适用于NextSeq 2000/2000-CN系统。

保质期

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
条目 储存温度 保质期
卡盒 -25°C至-15°C 6个月
流动槽 2°C至8°C 6个月
RSB(含Tween 20)* 2°C至8°C 6个月

* 室温下运输

每个流动槽通过过滤的read

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
流动槽类型 P1 P2 P3 *
单端reads 100M 400M 1.2B
双端reads 200M 800M 2.4B

* P3试剂仅适用于NextSeq 2000/2000-CN系统。
† 对于2 × 300 bp的测序深度,为300M单端reads。
对于2 × 300 bp的测序深度,600M双端reads。

质量分值*与运行时间

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
流动槽类型 P1 P2 P3
质量分值  
1 × 50 bp 在1 × 50 bp时,≥ 90%的碱基分值高于Q30
2 × 50 bp 在2 × 50 bp时,≥ 90%的碱基分值高于Q30
2 × 100 bp 在2 × 100 bp时,≥ 85%的碱基分值高于Q30
2 × 150 bp 在2 × 150 bp时,≥ 85%的碱基分值高于Q30
2 × 300 bp 在2 × 300 bp时,≥ 80%的碱基分值高于Q30
运行时间  
1 × 50 bp     约11小时
2 × 50 bp 约10小时 约13小时 约19小时
2 × 100 bp   约21小时 约33小时
2 × 150 bp 约19小时 约29小时 约48小时
2 × 300 bp 约34小时 约44小时  

* 质量分值(Q值)是对碱基检出中错误发生几率的预测。>Q30的碱基百分比根据整个运行中的数据取平均值。质量分值是基于NextSeq试剂盒在NextSeq 1000/1000-CN和NextSeq 2000/2000-CN系统上的运行所计算的,利用了Illumina PhiX对照文库。根据文库类型和质量、插入片段大小、上样浓度及其他实验因素的不同,性能表现可能有所差异。
† 运行时间包括NextSeq 1000/1000-CN或NextSeq 2000/2000-CN系统上的簇生成、测序和碱基检出。
‡ P3试剂仅适用于NextSeq 2000/2000-CN系统。

对关键应用估计的样本通量*

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
流动槽类型 P1 P2 P3
小型全基因组测序(300个循环)
130 Mb基因组;覆盖度大于30倍
~7 ~30 ~90
小型全基因组测序(600个循环)
130 Mb基因组;覆盖度大于30倍
~12 ~36
全外显子组测序(200个循环)
平均目标区域覆盖度为50倍;90%的目标区域覆盖度为20倍
~4 ~16 ~48
总RNA-Seq(200个循环) §
每个样本50M read对
~2 ~16 ~24
mRNA-Seq(200个循环)§
每个样本25M read对
~4 ~32 ~48
单细胞RNA-Seq(100个循环)**
5K细胞,20K reads/细胞
1†† ~4 ~11
miRNA-Seq或小RNA分析(50个循环)
11M reads/样本
~9‡‡ 36§§ ~108
16S RNA测序(600个循环) ~384*** ~384***

* 表格数据代表每次运行预计样本数。
P3试剂仅适用于NextSeq 2000/2000-CN系统。
使用P1 300个循环试剂盒。
§ 对于Illumina Stranded Total RNA Prep和Illumina Stranded mRNA Prep,建议读长是2 × 75 bp,对于Illumina RNA Prep with Enrichment建议读长是2 × 100 bp。
** 建议的测序深度在很大程度上取决于样本类型和实验目标,需要针对每项研究进行优化。
†† P1试剂是单细胞质量控制实验的好选择。
‡‡ 使用P1 100个循环试剂盒。
§§ 使用P2 100个循环试剂盒。
*** 因美纳提供最多384个唯一双标签序列。

Sequencing output per flow cella

NextSeq 1000 and NextSeq 2000
Flow cell type P1b P2b P3b,c P4c
1 × 50 bp       90 Gb
2 × 50 bp 10 Gb 40 Gb 120 Gb 180 Gb
2 × 100 bp   80 Gb 240 Gb 360 Gb
2 × 150 bp 30 Gb 120 Gb 360 Gb 540 Gb
2 × 300 bp 60 Gb 240 Gb    

a. Output specifications are based on an Illumina PhiX control library at supported cluster densities.
b. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.
c. P3 and P4 reagents are available for the NextSeq 2000 System only.

Shelf life

NextSeq 1000 and NextSeq 2000
Item Storage Temperature P1, P2, P3 Shelf Lifea P4 Shelf Lifeb
Cartridge -25°C to -15°C 6 months 3 months
Flow Cell 2°C to 8°C 6 months 3 months
RSB with Tween 20c 2°C to 8°C 6 months 6 months

a. P1, P2, and P3 with XLEAP-SBS chemistry will launch in Q2 of 2024 with a shelf life of 6 months.
b. In 1H of 2024, P4 shelf life will be 3 months. This will be extended to 6 months in 2H of 2024.
c. Shipped at room temperature.

Reads passing filter per flow cell

NextSeq 1000 and NextSeq 2000 with XLEAP-SBS chemistry
Flow Cell Type P1a P2a P3a,b P4b
Single-end Reads 100M 400M c 1.2B 1.8B
Paired-end Reads 200M 800M d 2.4B 3.6B

a. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.
b. P3 and P4 reagents are available for the NextSeq 2000 System only.
c. 300M single-end reads for 2 × 300 bp with standard SBS chemistry. 400M single-ends reads for 2 x 300 bp with XLEAP-SBS chemistry.
d. 600M paired-end reads for 2 × 300 bp with standard SBS chemistry. 800M single-ends reads for 2 x 300 bp with XLEAP-SBS chemistry.

Quality scores and run time

NextSeq 1000 and NextSeq 2000 with XLEAP-SBS chemistry
Flow cell type P1a P2a P3a,b P4b
Quality scoresc
1 × 50 bp ≥ 90% of bases higher than Q30
2 × 50 bp ≥ 90% of bases higher than Q30
2 × 100 bp ≥ 90% of bases higher than Q30
2 × 150 bp ≥ 90% of bases higher than Q30
2 × 300 bp ≥ 85% of bases higher than Q30
Run timed
1 × 50 bp       12 hr
2 × 50 bp 8 hr 12 hr 18 hr 20 hr
2 × 100 bp   19 hr 31 hr 34 hr
2 × 150 bp 17 hr 22 hr 40 hr 44 hr
2 × 300 bp 34 hr 42 hr    

a. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.
b. P3 and P4 reagents are available for the NextSeq 2000 System only.
c. Quality scores are based on an Illumina PhiX control library; performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
d. Run time includes cluster generation, sequencing, and base calling on a NextSeq 1000 or NextSeq 2000 System.

Estimated sample throughput for key applicationsa

NextSeq 1000 and NextSeq 2000 with XLEAP-SBS chemistry
Flow Cell Type P1b P2b P3b,c P4c
Small Whole-Genome Sequencing (300 cycles)
130 Mb genome; > 30× coverage
7 30 92 138
Whole-Exome Sequencing (200 cycles)
~8 Gb per exome, 90× mean coverage
~2d 10 30 45
Total RNA-Seq (200 cycles)
50M read pairs per sample
2d 8 24 36
mRNA-Seq (200 cycles)
25M read pairs per sample
4d 16 48 72
Single-Cell RNA-Seq (100 cycles)
5K cells, 20K reads/sample
1e 4 12 48
miRNA-Seq or Small RNA Analysis (50 cycles)
11M reads/sample
9f 36f 108f 163
16S RNA sequencing (600 cycles) 384g 384g    

a. Table values represent estimated number of samples per run. Recommended sequencing depth will largely depend on sample type and experimental objective and will need to be optimized for each study.
b. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.
c. P3 and P4 reagents are available for the NextSeq 2000 System only.
d. Use P1 300-cycle kit.
e. P1 reagents are a good option for single-cell quality control experiments.
f. Use 100-cycle kits.
g. A maximum of 384 unique dual indexes is available from Illumina.

 

 

 

 

 

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
仪器规格表

仪器配置
  • 具有集成式DRAGEN Bio-IT FPGA二级分析的独立干燥仪器
    仪器控制计算机
    • 主机:位于仪器内部的2U微型服务器
    • 内存:288 GB
    • 硬盘:3.8 TB SSD
    • 操作系统:Linux CentOS 7.6
    操作环境
    • 温度:15°C-30°C
    • 湿度:相对湿度20%–80%,非冷凝
    • 海拔:0–2000米
    • 仅供室内使用
    激光
    • 波长:449 nm、523 nm、820 nm
    尺寸
    • 宽x深x高:60 cm × 65 cm × 60 cm
    • 重量:141 kg
    包装尺寸
    • 包装宽×深×高:92 cm × 120 cm × 118 cm
    • 毛重:232 kg
    电源要求
    • 仪器输入电压:100 V到240 V交流电
    • 仪器输入频率:50/60 Hz
    网络连接带宽
    • 内部网络上传速度:200 Mb/s/仪器
    • BaseSpace Sequence Hub上传速度:200 Mb/s/仪器
    • 仪器操作数据上传速度:5 Mb/s/仪器
    产品安全与合规
    • 安全性:1级激光产品
    • NRTL认证IEC
    • 61010-1 CE认证
    • FCC/IC批准
  • 下载规格(PDF)
nextseq 2000 system drawing

 

 

 

 

 

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
卡盒

基于卡盒的易用、可持续性平台

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN测序系统利用了一种集成式卡盒,包括试剂、流体和废液容器,简化了文库上样和测序的操作。完成运行后,使用回收工具拆分试剂卡盒的多个塑料组件以便根据当地法规进行回收。

查看试剂

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN
流动槽

更高的产出,更低的运行成本

为了充分利用这些高密度流动槽,NextSeq 1000/1000-CN和NextSeq 2000/2000-CN测序系统专门配备了新型超高分辨率光学系统,与传统台式系统相比,该系统可生成分辨率和灵敏度更高的成像数据。微型化造就了可扩展的弹性同量,并且其数据质量与当前的NextSeq 550系统保持了相同的高标准。

查看数据一致性

NextSeq 2000 Cartidge
NextSeq 2000 Flow Cell

NextSeq 1000 and NextSeq 2000
Flow Cell

Increased output

To take full advantage of higher density flow cells, the NextSeq 1000 and NextSeq 2000 Sequencing Systems feature a novel super resolution optical system that yields highly accurate imaging data with greater resolution and higher sensitivity than traditional benchtop systems. This miniaturization provides scalability for a variety of output quantities while maintaining the same high standards of data quality enjoyed by NextSeq 550 System users today.

关键技术

边合成边测序技术

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN系统使用成熟的边合成边测序(SBS)技术产生的数据高度准确,性能可靠。SBS是所有因美纳测序平台都在采用的成熟技术。该技术支持大规模平行测序,使用专有的荧光标记可逆终止子方法。

了解更多

阵列式流动槽技术

NextSeq 1000/1000-CN和NextSeq 2000/2000-CN系统利用创新性阵列式流动槽技术为多种测序应用提供了出色的通量水平。阵列式流动槽在某些固定的位置包含数十亿个纳米井,这种设计甚至为待测序的簇之间提供了间隔距离。大幅增加了序列reads以及数据总输出。

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无损基因组数据压缩技术

无损基因组压缩可以在安装了NextSeq 1000/1000-CN和NextSeq 2000/2000-CN测序系统的仪器上使用。该技术使用超快速定位方法将reads定位到参考基因组,然后只存储重新生成这些reads所需的数据:一个位置和一个差异列表。

了解更多

想深入了解NextSeq 1000/1000-CN和NextSeq 2000/2000-CN的灵活应用?请立即咨询Illumina代表。

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Key Technology

Sequencing by Synthesis Technology

The NextSeq 1000 and 2000 systems leverage proven sequencing by synthesis (SBS) technology to to deliver highly accurate data and robust performance. SBS is the same proven technology used by all Illumina sequencing platforms. The technology supports massively parallel sequencing using a proprietary fluorescently labeled reversible terminator method. The NextSeq 2000 System is now powered by XLEAP-SBS chemistry with the new P4 flow cell.

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Patterned Flow Cell Technology

The NextSeq 1000 and 2000 systems utilize innovative patterned flow cell technology, which offers an exceptional level of throughput for diverse sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output.

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Lossless Genomic Data Compression Technology

Lossless genomic compression is available on-instrument with the NextSeq 1000 and NextSeq 2000 Sequencing Systems. This technology uses an ultra-fast mapping scheme to map reads onto a reference genome, and then store only the data needed to regenerate those reads: a position and a list of differences.

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Interested in learning more about the flexibility of NextSeq 1000/2000? Talk to an Illumina representative today.

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