The first time you process template DNA, try a concentration titration range to optimize the number of clusters formed. If the concentration is too low, fewer clusters are generated and result in a low sequencing yield. If the concentration is too high, clusters are too dense and can complicate data analysis. For more information, see the Sequencing Library qPCR Quantification Guide (requires a MyIllumina login) or the HiSeq Systems Denature and Dilute Libraries Guide.
For most libraries, Illumina recommends using a low-concentration
spike-in (1%) of PhiX Control v3 as an in-lane positive control for
alignment calculations and quantification efficiency.
No. The Illumina PhiX Control v3 is a separate product. The catalog number is: FC-110-3001.