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The NextSeq System comes with a region-specific power cord. For more information, see the NextSeq System Site Prep Guide.
Yes. The NextSeq reagent cartridge features reservoirs specifically for Read 1, Read 2, and index custom primers. See the NextSeq System Custom Primers Guide (document # 15057456).
The loading volume is 1.3 ml and the loading concentration is 1.8 pM.
NCS v1.2 or earlier requires a different loading volume and concentration.
For more information, see the NextSeq System Denature and Dilute Libraries Guide.
The number of libraries that can be run on a NextSeq are only limited by the number of indexes available. Multiplexing of libraries allows you to pool multiple samples together while the indexes enable you to pull the data back apart (de-multiplex). Multiplexed libraries are transferred onto the flow cell from a single reservoir on the NextSeq reagent cartridge.
Sequencing an insert size of 550 bp is supported with NextSeq v2 reagents. With NextSeq v1 reagents, an insert size of 350 bp is supported.
Yes. Using NextSeq 500/550 v2.5 reagents requires NextSeq Control Software (NCS) v2.2.0.
NextSeq 500/550 kits include reagents for paired-end sequencing and a paired-end flow cell. However, single-read runs are possible on a paired-end flow cell when the run is set up as a single-read run.
The NextSeq flow cell contains four physical lanes. However, libraries are loaded onto the flow cell from a single reservoir. You can sequence a single library or multiple pooled libraries on the flow cell.
There are two types of flow cells available for the NextSeq system: the high-output flow cell and the mid-output flow cell. Both flow cells contain four lanes of different width, resulting in a different number of tiles.
This chemistry allows us to ship the flow cells at ambient temperature. When received, store the flow cells at 2°C to 8°C.
Run duration depends on the type of flow cell and number of cycles. For example run times, see the data sheet for the NextSeq 550 Sequencing System. Run duration is the same for the NextSeq 500 system and the NextSeq 550 system.
Local Run Manager run mode - No sample sheet is required.
Manual run mode - A sample sheet is required in the following cases:
For NCS 2.2 or earlier, a sample sheet is only required when in standalone mode with converted FASTQ files and a third-party analysis software. Use Illumina Experiment Manager to create a sample sheet.
If data transfer is interrupted during a run, data are stored temporarily on the instrument computer until the connection is restored. When the connection is restored, transferring of data resumes automatically.
If the connection is not restored before the end of the run, data must be removed from the instrument computer manually before a subsequent run can begin.
NextSeq 550 Systems running NCS v4 or later can use Local Run Manager software modules for on-instrument analysis.
Additional analysis tools are available on BaseSpace Sequence Hub. You can also configure the NextSeq System to transfer data to a local server and perform analysis using third-party software.