Note
The software allows three attempts to successfully pass the flow check before allowing you to bypass the flow check. Bypassing the flow check can result in some lanes not clustering successfully.
After replacing the manifold—If the flow check failed a third time after replacing the manifold, you might have a clog in the hardware. Do the following to correct the problem:
After using the same manifold—If the flow check failed a third time and you have confirmed that you do not have a faulty manifold, you can now safely use the option to bypass the flow check. After the run, check reagent delivery from all tubes.
Problem |
Possible Cause |
Action |
Temperature out of range |
Potential Peltier or Peltier control board failure. |
Email Illumina Technical Support to schedule service. |
Coolant is flowing and coolant level is low |
Coolant has slowly evaporated to a low level. |
Add Illumina-supplied coolant (part # 1003709) to the coolant reservoir. |
Coolant is not flowing and coolant level is low |
Coolant level may be too low to generate flow. |
Add Illumina-supplied coolant (part # 1003709) to the coolant reservoir. |
Coolant is not flowing and coolant level is not low |
Potential coolant pump failure. |
Email Illumina Technical Support to schedule service. |
System is in a locked state |
Potential software error. |
Email Illumina Technical Support to schedule service. |