TruSight Tumor 15 Kit FAQs

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  • General


  • You can purchase standalone library prep reagents or a kit that includes both the library prep and sequencing reagents. The following kits are available:

    • Catalog # OP-101-1001–TruSight Tumor 15 MiSeq Kit (includes library prep and sequencing reagents) for 24 samples (48 libraries) with 3x MiSeq v3 reagents (600 cycle)
    • Catalog # 20005610–TruSight Tumor 15 MiniSeq Kit (includes library prep and sequencing reagents) for 24 samples (48 libraries) with 3x MiniSeq High Output reagents (300 Cycle)
    • Catalog # OP-101-1002–TruSight Tumor 15 (library prep only), for 24 samples (48 libraries)

    You can order an additional MiSeq reagent kit v3 (600 cycle) (MS-102-3003) or MiniSeq High Output reagents (300 cycle) (FC-420-1003).

    TruSight Tumor 15 targets 15 solid tumor genes, while TruSight Tumor 26 targets 26 genes. TruSight Tumor 15 is a multiplex, PCR amplicon-based assay and the TruSight Tumor 26 uses the TruSeq Custom Amplicon extension-ligation chemistry. 

    Expiration dates are printed on the kit box labels and reagent tubes. Illumina guarantees three months of shelf life from the time of kit shipment. 

    The Illumina Adapter Sequences Document lists index adapter sequences and all other adapter sequences.

    The following table lists the genes and exons included in the panel.

    Gene

    Target or Region

    Potential Disease States

    AKT1

    Exon 3 (partial)

    Breast

    BRAF

    Exon 15 (partial)

    Melanoma, Colon, Lung

    EGFR

    Focal Amplification, Exon 12 (partial),
    18, 19, 20, 21 (partial)

    Lung

    ERBB2

    Focal Amplification, Exons 14 (partial),
    17, 18, 19, 20 (partial), 21 (partial), 24, 26

    Breast, Lung

    FOXL2

    Exon 1 (partial)

    Ovary

    GNA11

    Exon 5 (partial)

    Melanoma

    GNAQ

    Exon 5 (partial)

    Melanoma

    KIT

    Exons 8, 9, 10, 11, 13, 14, 17, 18

    Gastric, Melanoma

    KRAS

    Exon 2 (partial), 3 (partial), 4

    Colon, Gastric, Lung

    MET

    Focal Amplification

    Lung, Colon, Gastric

    NRAS

    Exon 2 (partial), 3 (partial), 4

    Colon

    PDGFRA

    Exon 12, 14, 18

    Gastric, Melanoma

    PIK3CA

    Exon 10, 21

    Lung, Breast, Prostate

    RET

    Exon 16

    Lung

    TP53

    Full CDS

    Lung, Melanoma, Ovary, Colon

    Yes, this kit contains dual index adapters - 24 i7 indexes and 2 i5 indexes.

    The i7 index is 6 bp and i5 index is 8 bp. Although numbering of indexes seems similar to other kits, the index sequence is different and can be found in the reference guide.

    The output predefined report includes the following genes. These genes are also listed on the TruSight Tumor 15 product page.

    Gene

    Target or Region

    Potential Disease States

    AKT1

    E17K

    Breast

    BRAF

    V600E/K/R/M/D/G

    Melanoma, Colon, Lung

    EGFR

    Exon 19 and  Exon 20-insertions, deletions,
    and indels  G719A/C/S, L858R, L861Q,
    S7681, T790M

    Lung

    ERBB2

    p.E770_A771insAYVM

    Breast, Lung

    FOXL2

    C134W

    Ovary

    GNA11

    Q209L

    Melanoma

    GNAQ

    Q209L

    Melanoma

    KIT

    Exons 9, 11, 13, 14, 17

    Gastric, Melanoma

    KRAS

    Codons 12, 13, 19, 59, 61, 117, 146

    Colon, Gastric, Lung

    MET

    N/A

    Lung, Colon, Gastric

    NRAS

    Codons 12, 13, 59, 61, 117, 146

    Colon

    PDGFRA

    Exons 12, 14, 18

    Gastric, Melanoma

    PIK3CA

    Exons 9, 20

    Lung, Breast, Prostate

    RET

    M918T

    Lung

    TP53

    Full CDS

    Lung, Melanoma, Ovary, Colon