Input Requirements

Input Requirements

The VeriSeq PGS solution begins with DNA extraction and amplification from a single embryonic cell or multiple embryonic cells using the SurePlex DNA Amplification Kit. The VeriSeq PGS Library Preparation Kit protocol is optimized for 1 ng of input SurePlex amplified DNA. Illumina strongly recommends quantifying the starting SurePlex amplified dsDNA.

Input DNA Quantitation

VeriSeq PGS Library Preparation uses an enzymatic DNA fragmentation step and thus can be more sensitive to DNA input compared to mechanical fragmentation methods. The ultimate success of the assay strongly depends on using an accurately quantified amount of input DNA library. Therefore, the correct quantitation of the DNA library is essential. To obtain an accurate quantification of the DNA library, quantify the starting DNA library using a fluorometric based method specific for duplex DNA such as the Qubit dsDNA HS Assay system. Avoid methods that measure total nucleic acid content (e.g. nanodrop or other UV absorbance methods) because common contaminants such as ssDNA, RNA, and oligos are not substrates for the VeriSeq PGS assay.