Next-generation sequencing (NGS) library preparation involves generating a collection of DNA fragments for sequencing. NGS libraries are typically prepared by fragmenting a genomic DNA or cDNA sample and ligating specialized adapters to both fragment ends. An alternative process called "tagmentation" combines the fragmentation and ligation reactions into a single step. Adapter-ligated fragments are then PCR-amplified and gel-purified.
High-quality library preparation is critical for a successful NGS experiment. Illumina offers optimized sequencing library preparation solutions for a wide variety of NGS methods, including whole-genome sequencing, targeted DNA sequencing, whole-transcriptome sequencing, targeted RNA sequencing, and more.
Illumina library prep protocols can accommodate a range of throughput needs, from lower-throughput protocols for small laboratories to fully automated library preparation workstations for large laboratories or genome centers. Our solutions support a broad range of sample types, from cell lines to fresh tissue, formalin-fixed paraffin-embedded (FFPE) samples, blood, and other challenging sample types.
Illumina offers an extensive assortment of easy-to-use next-generation sequencing library preparation kits for DNA, RNA, and epigenetic sequencing studies. To find the right kit for your needs, use the selection tool below, or view a filterable list of library prep kits.Library Prep Kit Selector
For labs preparing large quantities of NGS libraries, liquid-handling robots and additional automation solutions provide high-throughput capacity and important quality control touch points. Visit the page below to learn more about Illumina-qualified sequencing library prep automation methods available through our partners.See Automation Options
This guide will walk you through the range of automation tools that exist for NGS library preparation, and help you find the right solution for your needs.Read Buyer's Guide
Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.
Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.
Find out how to quantify and validate final libraries for a successful sequencing run.
Learn when library normalization is required, and how to perform the necessary steps.
Learn how to use the PhiX library as a control in your Illumina sequencing runs.
Simultaneous qualification and quantification of nucleic acids with the Fragment Analyzer.
Access a summary of NGS methods compiled from peer-reviewed publications. Explore the wide range of questions that can be addressed with Illumina technology. Find method descriptions as well as pros and cons.
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