Illumina microarray technology (also known as BeadArray technology) uses silica microbeads. On the surface of each array, or BeadChip, hundreds of thousands to millions of genotypes for a single individual can be assayed at once. These tiny silica beads are housed in carefully etched microwells and coated with multiple copies of an oligonucleotide probe targeting a specific locus in the genome.
As DNA fragments pass over the BeadChip, each probe binds to a complementary sequence in the sample DNA, stopping one base before the locus of interest. Allele specificity is conferred by a single base extension that incorporates one of four labeled nucleotides. When excited by a laser, the nucleotide label emits a signal. The intensity of that signal conveys information about the allelic ratio at that locus.
Trusted data quality and exceptional coverage of valuable genomic regions make our Infinium genotyping arrays the platform of choice by leading institutions for high-throughput screening and large-scale research programs. Infinium technology produces exceptional data quality and call rates, as well as consistent reproducibility. We offer a broad range of microarray options based on SNP complexity and sample throughput.
Infinium methylation technology allows highly accurate and precise quantification of methylation levels in the genome. Use the Infinium methylation assay to detect cytosine methylation at the level of single CpG sites.
Anu Acharya describes why the combination of low cost and high quality led Mapmygenome to choose Illumina microarrays.
Researchers use genome-wide association studies to better understand the genetic risk loci associated with depression.
Using Infinium microarrays, GeneSeek achieves average call rates > 99.3% and 99.9% concordance.
Wojcik, G.L., Graff, M., Nishimura, K.K. et al. Genetic analyses of diverse populations improves discovery for complex traits. Nature. 2019;570(7762),514–518.
Capper, D., Jones, D., Sill, M. et al. DNA methylation-based classification of central nervous system tumours. Nature. 2018;555(7697),469–474.
Learn about the different strand designations found in Illumina array manifests and GenomeStudio projects.
A step-by-step method to help you understand this nomenclature for single nucleotide polymorphisms.
When comparing genotyping data, it is important to use the same DNA strand designation.
Illumina microarrays offer high-quality data and exceptional genomic coverage to propel genomic studies of any size.
Human genotyping arrays are ideal for processing thousands of samples to identify variants associated with traits and disease.
Methylation arrays enable high-throughput, quantitative interrogation of methylation sites across the genome at single-nucleotide resolution.
Software tools for array experimental design, sample tracking, and analysis of microarray data.