Preimplantation genetic screening (PGS) can determine the chromosomal ploidy of an embryo by screening the number of chromosomes prior to transfer in an in vitro fertilization cycle. The VeriSeq PGS Solution takes advantage of next-generation sequencing (NGS) technology to provide comprehensive, accurate screening of all 24 chromosomes for selection of embryos most likely to be euploid.

This bulletin details helpful resources for each step of the VeriSeq PGS workflow, from planning to report generation.

Figure 1. Overview of VeriSeq PGS workflow.

Planning: BlueFuse Workflow Manager for worklist and sample sheet setup

BlueFuse Workflow Manager User Guide
Instructions for using the BlueFuse Workflow Manager with VeriSeq PGS.

BlueFuse Workflow Manager Quick Reference Card
Instructions for using the BlueFuse Workflow Manager to create VeriSeq PGS worklists and sample sheets.

DNA Amplification

Single Cell Washing and Handling
Provides guidance for handling single cells and preparing them for the DNA amplification.

SurePlex Summary Protocol Experienced User Card
Experienced-level instructions for preparing samples using the SurePlex DNA Amplification kit.

VeriSeq PGS Library Prep Input Requirements
Recommendations for quantification methods for input into VeriSeq PGS.

Library Preparation

VeriSeq PGS Library Prep Reference Guide
Contains everything a first-time user needs, including an overview of the VeriSeq PGS Library Prep protocol, tips and techniques, protocol steps, kit contents, and user-supplied consumables.

VeriSeq PGS Library Prep Best Practices
Best practices for ensuring consistency, avoiding cross-contamination, and handling magnetic beads during library preparation.

VeriSeq PGS Library Prep Consumables & Equipment
List of consumables and equipment used with the VeriSeq PGS Library Prep Kit.
Note: Download and unzip before using.

Sequencing

VeriSeq PGS Solution MiSeq Data Sheet
Provides an overview of the VeriSeq PGS solution and details of recent field literature on the uses and applications of VeriSeq PGS.

MiSeq Reagent Kit v3-PGS Reagent Prep Guide
Provides an overview of kit contents and instructions for preparing sequencing reagents for a VeriSeq PGS run on the MiSeq instrument.

VeriSeq PGS MiSeq QC Assessment Guide – Technical Note
Provides guidance on the quality control metrics required for the VeriSeq PGS-MiSeq application.
Note: The metrics in this technical note are guidance and are not considered Illumina specifications.

Using the VeriSeq PGS-MiSeq in Low-Throughput Mode
Guidelines for running the VeriSeq PGS assay with fewer than 24 samples.

Data Analysis and Report Generation

BlueFuse Multi Software Guide
Reference guide for the BlueFuse Multi Software.

BlueFuse Multi Analysis Software for Preimplantation Genetic Screening
A sophisticated multiuser database for storing, analyzing, and interpreting results from preimplantation genetic screening studies.

VeriSeq PGS Technical Guide to Aneuploidy Calling
An introduction and guidelines for copy number calling for VeriSeq PGS samples using BlueFuse Multi software.

Troubleshooting

Identification of Contamination in PGS Samples
Recommendations for how to identify contamination in VeriSeq PGS data.

Modifying sample sheet information and requeueing VeriSeq PGS analyses in MiSeq Reporter - Bulletin
Instructions for modifying information in a VeriSeq PGS sample sheet and requeueing run data for secondary analysis in MiSeq Reporter.

Additional Support Links

VeriSeq PGS Library Prep Kit Support

BlueFuse Workflow Manager Support

BlueFuse Multi Software Support

VeriSeq Product Information Sheet

Online Training Courses

Product Pages

SurePlex DNA Amplification System

VeriSeq PGS Library Prep Kit