A comprehensive list of resources needed to set up, operate, maintain, and troubleshoot the iSeq 100 is on the iSeq 100 Support page. Specific documentation links can be found below.

• System Setup Poster
  Contains information for first-time instrument setup. It is included with the shipment of the instrument.
• Illumina CMOS Chip and One-Channel SBS Chemistry tech note
  Provides an explanation of single-channel chemistry and how it compares to two- and four-channel chemistries.
• QC and rebalancing of single-cell gene expression libraries using the iSeq 100 System
  Assess key metrics of multiplexed single-cell libraries to evaluate quality and enable accurate rebalancing before high-depth runs on the NextSeq 550 or NovaSeq 6000 Systems.
• Sequencing Library QC with the iSeq 100 System
  This application note details how to use the iSeq 100 System to sequence libraries as a quality control (QC) step before high throughput sequencing.
• iSeq 100 System Specification Sheet
  Contains basic information including output and time specifications for the iSeq100.
• iSeq 100 System Specification Page
  Provides the data quality and yield specifications for the iSeq 100.
• iSeq 100 Sequencing System Site Prep Guide
  Contains lab requirements, networking guidelines, and security considerations.
• iSeq 100 Sequencing System Guide
  Contains dilution and loading instructions, a workflow overview, maintenance procedures, troubleshooting resources, software restriction policy information, and FAQs.
• Online Training Courses
  Includes video courses on unboxing and installing your instrument, how to start a run, and setting up / viewing analysis results on Local Run Manager. To access additional online training courses, go to Illumina.com -> Support -> Webinars and Trainings -> Online Courses -> Sequencing.
• Safety Data Sheets
  Safety Data Sheets contain information to help with appropriate reagent disposal practices. To obtain this information, enter the kit catalog number, language, and region/country, then select Search.

Additional technical resources are available 24/7 at the iSeq 100 Sequencing System support website.