04/07/22
Illumina offers a wide array of library preparation kits and sequencing platforms for different applications, each with its own coverage and indexing considerations. The table below lists the Illumina sequencing primers and library preparation kits compatible with the iSeq 100 platform based on the required data output*, read length, and the indexing capabilities of each kit.
Note: Most Illumina libraries can be sequenced with all Illumina sequencing platforms. If a specific Illumina library is not listed below, contact Illumina Tech Support to discuss special considerations.
Refer to the Sequence Coverage Calculator and Library Prep and Array Kit Selector resources for additional information.
Table 1. Compatible sequencing primers and recommended library types for the iSeq 100 sequencing platform.
| iSeq100 | ||||
|---|---|---|---|---|
| Read 1 Primer |
Index 1 (i7) Primer |
Index 2 (i5) Primer |
Read 2 Primer |
Library Type |
| HP10 | HP14 | HP14 | HP11 | AmpliSeq Panels Illumina DNA Prep (M) Tagmentation Illumina DNA Prep with Enrichment (with small panels) Nextera Rapid Capture Custom Enrichment Nextera XT DNA TruSeq ChIP TruSeq DNA PCR-Free TruSeq Nano DNA TruSeq RNA v2 TruSeq Small RNA TruSeq Targeted RNA Expression Panels TruSight Cancer TruSight Cardio TruSight Inherited Disease TruSight Myeloid TruSight Rapid Capture TruSight RNA Fusion TruSight RNA Pan-Cancer TruSight Tumor 15 |
*The iSeq 100 sequencing platform has a maximum data output of 1.2 Gb.