Illumina adapter portfolio
08/11/20
The first step of sequencing is to construct a library from DNA or RNA. A library contains DNA inserts flanked on each side by an adapter, as shown in Fig. 1:
Figure 1: Schematic representation of a dual-indexed library fragment
Adapters contain:
- Sequences that allow the library to bind and generate clusters on the flow cell (p5 and p7 sequences)
- Sequencing primer binding sites to initiate sequencing (Rd1 SP and Rd2 SP)
- Index sequences (Index 1 and, where applicable, Index 2), which are sample identifiers that allow multiplexing/pooling of multiple samples in a single sequencing run or flow cell lane.
Illumina offers a wide range of adapter kits to allow flexibility and multiple indexing strategies. Illumina adapter kits differ from one another based on the number of samples they support, the length and sequences of the indexes, as well as the chemistry by which they attach to the insert fragment.
The tables below list the product specifications of the Illumina adapter kits:
- Plexity refers to the number of unique index sequences per kit
- Index Lengths refers to the number of cycles of sequencing required to read Index 1 and Index 2, respectively
| Ligation Based Adapters | ||||
|---|---|---|---|---|
| Adapter | Characteristics | Plexity | Index Lengths | Compatible Library Prep kits |
|
TruSeq™ DNA/RNA Single Indexes Set A and B |
Single Indexes (Tubes) |
24 (12 per set) |
6 + 0 | Nextera Mate Pair |
| TruSeq ChIP-Seq | ||||
| TruSeq DNA Nano | ||||
| TruSeq DNA PCR Free | ||||
| TruSeq DNA Exome | ||||
| TruSeq Methyl Capture EPIC (Set A only) | ||||
| TruSeq RNA Exome | ||||
| TruSeq RNA V2 | ||||
| TruSeq Stranded mRNA | ||||
| TruSeq Stranded Total RNA | ||||
| TruSight RNA Fusion | ||||
| TruSight RNA PanCancer | ||||
| TruSeq DNA/RNA CD Indexes |
Combinatorial Dual Indexes (Plate) |
96 | 8 + 8 | TruSeq DNA Exome |
| TruSeq DNA Nano | ||||
| TruSeq DNA PCR Free | ||||
| TruSeq Stranded mRNA | ||||
| TruSeq Stranded Total RNA | ||||
|
IDT for Illumina – TruSeq DNA/RNA UD Indexes (and v2) |
Unique Dual Indexes (Plate) |
24 or 96 | 8 + 8 | TruSeq DNA Exome |
| TruSeq DNA Nano | ||||
| TruSeq DNA PCR Free | ||||
| TruSeq Stranded mRNA | ||||
| TruSeq Stranded Total RNA | ||||
|
TruSight Tumor 170 and TruSight Oncology Indexes |
Combinatorial Dual Indexes (DNA), Unique Dual Indexes (RNA) (Tubes/Plate) |
16 | 8 + 8 | TruSight Oncology 500 |
| TruSight Tumor 170 | ||||
|
Unique Dual Indexes (Tubes) |
16 | 8 + 8 | TruSight Oncology ctDNA | |
|
AmpliSeq CD Indexes Set A-D for Illumina |
Combinatorial Dual Indexes (Plate) |
384 (96 per set) |
8 + 8 | AmpliSeq Panels |
| AmpliSeq UD Indexes for Illumina |
Unique Dual Indexes (Plate) |
24 | 8 + 8 | AmpliSeq Panels |
| PCR Based Adapters | ||||
|---|---|---|---|---|
| Adapter | Characteristics | Plexity | Index Lengths | Compatible Library Prep kits |
| Nextera™ DNA CD Indexes |
Combinatorial Dual Indexes (24 Index - Tubes, 96 Index - Plate) |
24 or 96 | 8 + 8 | Nextera DNA Exome |
|
Illumina DNA Prep, (M) Tagmentation1 |
||||
| Nextera XT Index Kit v2 Set A-D |
Combinatorial Dual Indexes, compatible with bead-based normalization (BBN) (Tubes) |
384 (96 per set) |
8 + 8 | Nextera XT |
| Nextera XT Index Kit |
Combinatorial Dual Indexes, compatible with bead-based normalization (BBN) (Tubes) |
24 | 8 + 8 | Nextera XT |
| TruSeq Custom Amplicon Index Kit |
Combinatorial Dual Indexes, compatible with bead-based normalization (BBN) (Tubes) |
96 | 8 + 8 | TruSight Myeloid Sequencing Panel |
| R7XX/A5XX index kits |
Combinatorial Dual Indexes (Tubes) |
24 | 6 + 8 | TruSight Tumor 15 |
|
48 or 384 (96 per set) |
6 + 8 | TruSeq Target RNA Expression | ||
| TruSight™ Rapid Capture Kits |
Combinatorial Dual Indexes (Tubes) |
1,2,4,24 or 96 | 8 + 8 | TruSight Cancer |
| TruSeq Neurodegeneration | ||||
| TruSight Cardio | ||||
| TruSight Inherited Disease | ||||
| TruSight One | ||||
| TruSight Rapid Capture | ||||
|
IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation3 |
Unique Dual Indexes (Plate) |
384 (96 per set) | 10 + 10 |
Illumina RNA Prep with Enrichment, (L) Tagmentation |
|
Illumina DNA Prep, (M) Tagmentation1 |
||||
|
Illumina DNA Prep with Enrichment, (S) Tagmentation2 |
||||
| Nextera XT | ||||
|
Illumina DNA PCR-Free Prep, Tagmentation4 |
||||
| Ligation and PCR Based Adapters | ||||
|---|---|---|---|---|
| Adapter | Characteristics | Plexity | Index Lengths | Compatible Library Prep kits |
| TruSeq Small RNA index Kit Set A-D |
Single Indexes (adds RNA adapter by ligation, adds indexes by PCR) (Tubes) |
48 (12 per set) | 6 + 0 | TruSeq Small RNA |
|
IDT for Illumina RNA UD Indexes Set A-D, Ligation |
Unique Dual Indexes (adds Anchors by ligation, adds indexes by PCR) (Plate) |
384 (96 per set) |
10 + 10 | Illumina Stranded mRNA Prep, Ligation |
|
Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus |
||||