08/22/2022
To offer flexibility to customers, Illumina offers library preparation and index kits in a decoupled format, meaning that the index kits are sold separately from the library preparation kits. This format is intended to offer a range of indexing possibilities. This bulletin lists the library preparation kits which have multiple indexing strategies, and the recommended index kits. This will help customers choose the right index kit option suitable for their project.
In May 2020, Illumina announced changes to some of the IDT for Illumina indexes; the new names and catalog numbers are provided in this bulletin.
Library preparation kits that have not been decoupled are not listed in this bulletin.
For more information on each index kit please refer to the Illumina adapter portfolio support bulletin.
| Library Prep Kit | Index Kit Name | Index Kit Catalog # | New Index Kit Name | New Catalog # |
|---|---|---|---|---|
| TruSeq DNA Nano TruSeq PCR Free |
TruSeq DNA Single Indexes Set A & B (12 Indexes, 24 Samples per set) |
A: 20015960 B: 20015961 |
||
| TruSeq DNA CD Indexes (96 Indexes, 96 Samples) |
20015949 | |||
| IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples) |
20020590 | |||
| IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples) |
20022370 | IDT for Illumina - TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples) |
20040870 | |
| TruSeq Stranded Total RNA TruSeq Stranded mRNA |
TruSeq RNA Single Indexes Set A & B (12 Indexes, 48 Samples per set) |
A: 20020492 B: 20020493 |
||
| TruSeq RNA CD Index Plate (96 Indexes, 96 Samples) |
20019792 | |||
| IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples) |
20020591 | |||
| IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples) |
20022371 | IDT for Illumina - TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples) |
20040871 | |
| Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Stranded mRNA Prep, Ligation |
IDT for Illumina RNA UD Indexes Set A-D, Ligation (96 Indexes, 96 Samples per set) |
A: 20040553 B: 20040554 C: 20040555* D: 20040556* |
||
| Illumina RNA Prep with Enrichment, (L) Tagmentation |
IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20042666 D: 20042667 |
||
| TruSeq Targeted RNA Expression | TruSeq Targeted RNA Index Kit (48 Indexes, 48 Samples) |
RT-401-1001 | ||
| TruSeq Targeted RNA Index Kit A-D (96 Indexes, 96 Samples per set) |
A: RT‑402‑1001 B: RT‑402‑1002 C: RT‑ D: RT‑402‑1004 |
|||
| Illumina DNA PCR-Free Prep, Tagmentation |
IDT for Illumina Nextera DNA UD Indexes Set v1 A-B (96 Indexes, 96 Samples per set) Set v2 C-D |
A: 20027213 B: 20027214 C: 20042666 D: 20042667 |
IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20042666 D: 20042667 |
| Illumina DNA Prep, (M) Tagmentation [Formerly Nextera DNA Flex] |
IDT for Illumina Nextera DNA UD Indexes Set A-D (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20027215 D: 20027216 |
IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20042666 D: 20042667 |
| Nextera DNA CD Indexes (24 Indexes, 24 Samples) |
20018707 | |||
| Nextera DNA CD Indexes (96 Indexes, 96 Samples) |
20018708 | |||
| Illumina DNA Prep with Enrichment, (S) Tagmentation [Formerly Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents] |
IDT for Illumina Nextera DNA UD Indexes Set A-D (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20027215 D: 20027216 |
IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20042666 D: 20042667 |
| IDT for Illumina Nextera DNA UD Indexes Sets A-D (384 Indexes, 384 Samples) |
20027217 | |||
| Nextera XT DNA | IDT for Illumina Nextera DNA UD Indexes Set A-D (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20027215 D: 20027216 |
IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set) |
A: 20027213 B: 20027214 C: 20042666 D: 20042667 |
| Nextera XT Index Kit v2 Set A-D (96 Indexes, 384 Samples per set) |
A: FC‑131‑2001 B: FC‑131‑2002 C: FC‑131‑2003 D: FC‑131‑2004 |
|||
| Nextera XT Index Kit (24 Indexes, 96 Samples) |
FC-131-1001 | |||
| AmpliSeq for Illumina library prep PLUS kits | AmpliSeq UD Indexes for Illumina (24 Indexes, 24 Samples) |
20019104 | ||
| AmpliSeq CD Indexes Set A-D for Illumina (96 Indexes, 96 Samples per set) |
A: 20019105 B: 20019106 C: 20019107 D: 20019167 |
|||
| AmpliSeq CD Indexes Sets A-D for Illumina (384 Indexes, 384 Samples) |
20031676 | |||
| AmpliSeq CD Indexes Large Volume for Illumina (96 Indexes, 96 Samples) |
20019108 |
* Not yet released