Illumina NGS (Next Generation Sequencing) platforms require a post run wash and/or maintenance wash to be performed with regularity (refer to the appropriate instrument guide for details). To avoid contaminating the fluidics lines during these washes, Illumina recommends following these best practices.

1. After removing the wash cartridge from the instrument, properly discard any remaining wash solution (eg, do not reuse Tween wash solution).
2. Thoroughly rinse each position of the wash cartridge with lab grade warm water.
3. Place the wash cartridge upside down with some space to allow air flow and proper drying.
4. Inspect the wash cartridge wells regularly for contamination (refer to Fig. 1, black or pink discoloration may be noted).

Figure 1. Black ring at bottom of wash cartridge well.

1. Prepare a 1–3% lab grade bleach solution to fill the wells of the tray.
2. Use a brush to remove the mold from the wells.
3. IMPORTANT: Thoroughly rinse the wells (at least three times) with DI or lab grade water (residual bleach can cause clustering issues on subsequent runs).
4. Place cartridge upside down with some space to allow air flow and proper drying.
5. Repeat as necessary if mold is still present.
6. If it is not possible to remove all the mold, contact Illumina Technical Support to discuss replacing the wash cartridges.

For step-by-step guidance, please refer to this Video link

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