Illumina DNA Prep with Enrichment (formerly Nextera Flex for Enrichment) is a targeted enrichment workflow that is compatible with both Illumina and third-party enrichment panels.

Third-Party DNA Probe Panel Requirements

If using third-party biotinylated DNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:

• 80 bp or 120 bp probe length
• Between 500 and 675,000 probes
• Single-stranded or double-stranded DNA probes
• Total probe input of ≥ 3 pmole for enrichment at plexities from 1-plex to 12-plex

Follow the Illumina DNA Prep with Enrichment Reference Guide when using DNA probes.

Third-Party RNA Probe Panel Requirements

If using third-party biotinylated RNA probe panels with the Illumina DNA Prep with Enrichment workflow, the enrichment panels need to meet the following criteria:

• 120 nt probe length
• Between 300,000 and 675,000 probes
• Single-stranded
• Total probe input of ≥ 3 pmoles for enrichment at plexities from 1-plex to 12-plex

Follow the Nextera Flex for Enrichment for RNA Probes Demonstrated Protocol when using RNA probes.

The use of third-party probe panels with Illumina DNA Prep with Enrichment may require additional optimization and validation, because third-party probe panels have not been validated by Illumina.