Final library trace examples for Illumina Library Prep Kits
10/27/21
When preparing Illumina sequencing libraries, we recommend performing quality control analysis on the final library using the Agilent Bioanalyzer, Fragment Analyzer, or similar instruments to assess the library size and profile. The following library sizes are guides, and profiles may vary slightly. For more information, see the appropriate library prep kit reference guide.
Illumina DNA Library Prep Kits
| Kit name | Size in bp (range) | Trace |
Illumina DNA Prep (M) Tagmentation |
600 (150–1500) |
 |
Illumina DNA Prep with Enrichment (S) Tagmentation |
350 (200–1000) |
 |
Nextera DNA Library Prep Kits
| Kit name | Size in bp (range) | Trace |
Nextera XT DNA Library Prep Kit |
(~250–1000) |
 |
Illumina RNA Library Prep Kits
| Kit name | Size in bp (range) | Trace |
TruSeq DNA Nano |
Average insert size: 350 |
 |
Average insert size: 550 |
 |
|
TruSeq DNA PCR-Free |
Average insert size: 350 |
 |
Average insert size: 550 |
 |
|
TruSeq DNA Exome |
(~200–400) |
 |
TruSeq ChIP
|
(~250–300) |
 |
TruSeq Methyl Capture EPIC |
(~250–1000) |
|
Illumina RNA Library Prep Kits
| Kit name | Size in bp (range) | Trace |
Illumina Stranded mRNA |
~300–400 |
 |
Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus |
~375–475 |
 |
Illumina RNA Prep with Enrichment (L) Tagmentation |
~380–450 |
 |
TruSeq RNA Library Prep Kits
| Kit name | Size in bp (range) | Trace |
TruSeq stranded mRNA
TruSeq stranded Total RNA
TruSeq RNA Sample Preparation v2 |
~260 |
 |
TruSeq RNA Exome
|
~250–300 A larger fragment size is expected for good FFPE RNA (> 350 bp), while a smaller fragment size is expected for poor FFPE RNA. |
 |
TruSeq small RNA |
(145–160) |
 |
TruSeq Targeted RNA Expression Kit |
Library size will depend on the target amplicon size of the panel and the input RNA quality. |
Intact RNA FFPE RNA  |
TruSight Library Prep Kits
| Kit name | Size in bp (range) | Trace |
TruSight Tumor 15
|
~350 |
|
TruSight Cancer TruSight Rapid Capture
|
(~200–1000) |
 |
TruSight RNA Fusion TruSight RNA Pan-Cancer
|
(~200–1000) |
 |
AmpliSeq for Illumina Library Prep Kits
| Kit name | Size in bp (range) | Trace |
BRCA Panel
Cancer Hotspot Panel v2
Comprehensive Cancer Panel
|
(201–274)
(186–277)
(186–323) |
 |
Childhood Cancer Panel*
Comprehensive Panel v3†
Focus Panel‡
|
DNA library: (*220–340) (†186–305) (‡197–277) |
|
RNA library: (*175–400) (†170–446) (‡170–284) |
|
|
Exome Panel |
(292–376) |
 |
Immune Repertoire Plus, TCR beta Panel |
Library size may vary based on the input sample. |
 |
Myeloid Panel
|
DNA library: (200–550) |
 |
RNA library: (181–373) |
 |
|
Immune Response Panel
Transcriptome Human Gene Expression Panel
|
(215–274)
(202–267) |
 |
TCR beta-SR Panel |
DNA library: N/A |
N/A |
RNA library: Library size may vary based on the input sample. |
 |
