HiSeq 1500 Documentation

System overview and instructions for operating and maintaining the HiSeq 1500.

Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the HiSeq 2500, HiSeq 1500, or HiSeq 2000 System.

Instructions for sequencing with custom primers on any HiSeq system (except the HiSeq X System).

The safety and compliance guide describes safety considerations and lists product compliance markings for the HiSeq 1500 and HiSeq 2500 systems.

Primer rehybridization instructions using HiSeq v4 or TruSeq v3 chemistry.

Instructions for performing primer rehybridization on the HiSeq using the HiSeq Rapid Rehybridization Kit or the TruSeq Rapid Rehybridization Kit.

Instructions for using the TruSeq v2 cBot Multi-Primer Re-Hybridization Kit for primer rehybridization on a TruSeq v3 Flow Cell.

This guide contains instructions for sequencing dual-indexed libraries on the HiSeq.

The sequencing process for single- and dual-indexed runs on Illumina instruments.

Guidelines for managing the security of the control computer on your sequencing system. Use these guidelines to configure your system and ensure a more secure operating environment.

It is strongly recommended that you review this documentation to keep your instrument secure.

This site is updated frequently. Make sure to visit this site often for the latest security recommendations.