These free self-paced courses guide you through setting up and getting started with your MiSeq i100 system. If you are new to Illumina and next-generation sequencing, see the
NGS for beginners page for additional resources.
The Illumina service and support team offers paid services for instrument installation, qualification, and training. Contact your sales representative for more information.
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Course duration: 20 minutes | This course shows you how to set up your MiSeq i100 Series system. Topics include preparing your lab space, identifying the test components, and performing first time setup. (English revised October 2025. Other languages revised November 2024.)
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Course duration: 25 minutes | Learn how to start a run on the MiSeq i100 Series. This course introduces the options for planning a run and analyzing sequencing data, consumable preparation, and how to use the MiSeq i100 Series Control Software to initiate the run. (English revised October 2025. Other languages revised January 2025.)
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Course duration: 20 minutes | Find out about the options for monitoring and assessing your MiSeq i100 Series run, including the available options, where to find key metrics, and some troubleshooting steps if your run does not meet expectations. (English revised October 2025. Other languages revised January 2025.)
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Course duration: 20 minutes | Illumina DNA prep is a fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications. Topics in this course include the components of the kit, how the protocol works, what is needed to complete the protocol, and various steps in the library preparation workflow.
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Course duration: 30 minutes | Illumina DNA Prep with Enrichment is a complete targeted resequencing solution for a wide range of applications, with fast integrated workflow and options for custom and fixed enrichment panels. Learn about the kit and its components, how the protocol works, and various steps in the library preparation workflow. (English revised October 2025. Other languages revised November 2024.)
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Course duration: 30 minutes | Illumina RNA Prep with Enrichment is a fast, integrated workflow for producing enriched and indexed sequencing libraries from a broad range of sample types and RNA input quantities. This course describes the kit and its components, summarizes the steps of the protocol, and provides best practices. (English revised October 2025. Other languages revised November 2024.)
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Course duration: 25 minutes | This course provides an overview of the BCL convert workflow on the MiSeq i100 Series. Learn about the features of the workflow, steps in the workflow, and the steps to analyze data with the onboard DRAGEN BCL convert app. (English revised October 2025. Other languages revised January 2025.)
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Course duration: 25 minutes | This course provides an overview of the Library QC workflow on the MiSeq i100 Series. We'll cover the features of the Library QC workflow, the steps in the workflow, and review the onboard DRAGEN Library QC app analysis results. (English revised October 2025. Other languages revised January 2025.)
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Course duration: 30 minutes | This course provides an overview of the microbial enrichment panel analysis workflow with the MiSeq i100 Series. Topics covered include features of the workflow, steps in the workflow, the available enrichment panels, and the steps to analyze the data with the onboard DRAGEN Microbial Enrichment Plus app. (English revised October 2025. Other languages revised January 2025.)
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Course duration: 20 minutes | Learn about the Small Whole Genome Sequencing (Small WGS) workflow on the MiSeq i100 Series. We'll introduce features of the Small WGS workflow, describe the steps in the workflow, and review the results of the onboard DRAGEN Small WGS app. (English revised October 2025. Other languages revised January 2025.)
