Before preparing libraries, record information about your samples using an option available for the NextSeq 550 System.
The AmpliSeq for Illumina Comprehensive Cancer Panel provides a targeted resequencing solution for analyzing somatic mutations across 409 genes with known associations to cancer.
| Number of Pools | Concentration | Number of Amplicons | Average Amplicon Length (bp) | Average Library Length (bp) |
|---|---|---|---|---|
| 4 | 2X | 15,992 | 109 | 249 |
Completing the AmpliSeq for Illumina Comprehensive Cancer Panel protocol requires the panel and the following library prep and index kit. The AmpliSeq for Illumina Library PLUS Kit is available in 24-, 96-, and 384-reaction configurations.
Because the AmpliSeq for Illumina Comprehensive Cancer panel has four oligo pools, it requires more input DNA and reagents than panels with one or two pools.
For each DNA sample, the AmpliSeq for Illumina Comprehensive Cancer Panel generates one DNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. A library has the ends required for sequencing.
Preparing the appropriate number of samples requires the following number and combination of kits.
| Number of Samples | 24 Samples | 96 Samples | 384 Samples |
|---|---|---|---|
| Number of Libraries | 24 Libraries | 96 Libraries | 384 Libraries |
| AmpliSeq for Illumina Comprehensive Cancer Panel | 1 | 4 | 16 |
| AmpliSeq Library PLUS For Illumina | 2 24-reaction kits | 2 96-reaction kits | 2 384-reaction kits |
| AmpliSeq CD Set A (96 reaction, 96 indexes) | 1 | 1 | 4 |
| cDNA Synthesis | Not applicable | Not applicable | Not applicable |
Sequence AmpliSeq for Illumina Comprehensive Cancer Panel libraries using the following systems and reagent kits.
| System | Reagent Kit | Samples per Run |
|---|---|---|
| NextSeq System | NextSeq Mid Output v2 Kit | 4 |
| NextSeq High Output v2 Kit | 12 |