AmpliSeq for Illumina TCR beta-SR Panel Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system:

Library Prep

Panel Description

AmpliSeq for Illumina TCR beta-SR Panel is a highly multiplexed targeted resequencing panel intended for use with multiple sample types, including formalin-fixed paraffin-embedded (FFPE) tumor samples. 

Component Number of Pools Concentration
DNA and RNA 1 5X
Library Prep and Index Kits

Completing the AmpliSeq for Illumina TCR beta-SR protocol requires the panel and the following library prep kit and index kit.

Number of Samples

For each DNA and RNA sample, the AmpliSeq for Illumina TCR beta-SR Panel generates one DNA and one RNA library. A sample is a specimen of nucleic acid from one source, while a library is nucleic acids from a sample after preparation. Samples can be DNA, RNA, or paired. A library has the ends required for sequencing.

Preparing the appropriate number of samples requires the following number and combination of kits. 

Number of Samples 24 Samples 96 Samples 384 Samples
Number of Libraries 24 Libraries 96 Libraries 384 Libraries
AmpliSeq for Illumina TCR beta-SR Panel 1 5 16
AmpliSeq Library PLUS For Illumina 1 24-reaction kit (20019101) 1 96-reaction kit (20019102) 1 384-reaction kit (20019103)
AmpliSeq CD Set A (96 reaction, 96 indexes) 1 1 4
AmpliSeq UD Indexes for Illumina (24 Indexes, 24 Samples) 1 4 16
[RNA] cDNA Synthesis 1 1 4

Sequencing Guidelines

Sequence AmpliSeq for Illumina TCR beta-SR Panel libraries using the following systems and reagent kits.

System Reagent Kit
MiniSeq System MiniSeq Mid Output Reagent Kit
  MiniSeq High Output Reagent Kit
NextSeq System NextSeq Mid Output v2 Kit
  NextSeq High Output v2 Kit