Contents & Storage

The TruSeq Rapid Exome Library Prep Kit contains the following components and is shipped on dry ice unless specified otherwise below.

Each kit contains 3 boxes of reagents, 1 box of oligos, and 1 box of index replacement caps.

 

Box 1, Store as specified

Quantity per 8 rxn kit

 

 

 

1 plex

3 plex

6 plex

9 plex

12 plex

Reagent

Description

Storage Temperature

1

1

2

2

3

SPB

Sample Purification Beads

2°C to 8°C

4

4

4

4

4

SMB

Streptavidin Magnetic Beads

2°C to 8°C

1

1

1

1

1

ET2

Elute Target Buffer 2

2°C to 8°C

1

1

1

1

1

EHB2

Enrichment Hybridization Buffer 2

15°C to 30°C

1

1

1

1

1

ST

Stop Tagment Buffer

15°C to 30°C

 

Box 2, Store at -25°C to -15°C

Quantity per 8 rxn kit

 

 

1 plex

3 plex

6 plex

9 plex

12 plex

Reagent

Description

1

1

1

1

1

RSB

Resuspension Buffer

2

2

2

2

2

EEW

Enhanced Enrichment Wash Solution

1

1

2

3

1

TDE2

Tagment DNA Enzyme 2

1

1

1

1

1

EE1

Enrichment Elution Buffer 1

1

1

1

1

1

BLR

Blocker

1

1

2

3

2

LAM

Library Amplification Mix

1

1

1

1

1

EHB1

Enrichment Hybridization Buffer 1

1

1

1

1

1

HP3

2N NaOH

1

1

1

1

1

PPC

PCR Primer Cocktail

1

1

1

1

1

EAM

Enrichment Amplification Mix

1

1

1

2

2

TD

Tagment DNA Buffer

 

Box 3, Store at -25°C to -15°C

Quantity per 8 rxn kit

   

1 plex

3 plex

6 plex

9 plex

12 plex

Reagent

Description

1

1

2

3

E502

E502 Index Adapter

2

E505

E505 Index Adapter

2

E506

E506 Index Adapter

1

1

1

1

N701

N701 Index Adapter

1

1

1

1

N702

N702 Index Adapter

1

1

1

N703

N703 Index Adapter

1

1

1

1

N704

N704 Index Adapter

1

1

1

1

N705

N705 Index Adapter

1

N706

N706 Index Adapter

1

1

1

N707

N707 Index Adapter

1

N708

N708 Index Adapter

1

N709

N709 Index Adapter

1

1

N710

N710 Index Adapter

1

1

N711

N711 Index Adapter

1

1

N712

N712 Index Adapter

 

Oligos, Store at -25°C to -15°C

Quantity

Reagent

Description

4

CEX

Coding Exome Oligos

 

Index Adapter Replacements, Store at 15°C to 30°C

Quantity

Description

1

i7 Index Tube Caps, Orange

1

i5 Index Tube Caps, White