The NeoPrep System normalizes libraries to 10nM.
TruSeq Stranded mRNA Library Prep for NeoPrep libraries result in the following cluster densities:
Instrument |
Insert Size |
Predenature concentration (nM) |
Denatured/diluted intermediate concentration (pM) | Pooled denatured samples pM load | Expected Cluster Density (mm2) |
MiSeq
|
200 |
10 |
50 | 10 | 1200 |
NextSeq |
200 |
4 |
40 | 2–3 | ~200 |
HiSeq 2500 High Output v3
|
200 |
2 |
20 | 6–10 | 700–1000 |
HiSeq 2500 High Output v4
|
200 |
2 |
20 | 12–15 | 1250 |
HiSeq 2500 Rapid Run v1
|
200 |
2 |
20 | 6–10 | 600–1000 |