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TruSeq Stranded mRNA Contents & Storage

TruSeq Stranded mRNA Library Prep (48 Samples)

Box 1
Quantity Reagent Description Storage
1 RPB RNA Purification Beads 2°C to 8°C
1 DTE CTE Dilution Tube Room temperature
1 DTA CTA Dilution Tube Room temperature
1 DTL CTL Dilution Tube Room temperature
Box2
Quantity Reagent Description Storage
1 BBB Bead Binding Buffer 2°C to 8°C
1 BWB Bead Washing Buffer 2°C to 8°C
1 ELB Elution Buffer 2°C to 8°C
1 FPF Fragment, Prime, Finish Mix -25°C to -15°C
Core Library Prep Box 1, Store at -25°C to -15°C
Quantity Reagent Description
1 ATL A-Tailing Mix
1 CTA A-Tailing Control
1 CTE End Repair Control
1 CTL Ligation Control
1 LIG Ligation Mix
1 RSB Resuspension Buffer
1 STL Stop Ligation Buffer
Core Library Prep Box 2, Store at -25°C to -15°C
Quantity Reagent Description
1 PMM PCR Master Mix
1 PPC PCR Primer Cocktail
1 FSA First Strand Synthesis Act D Mix
1 SMM Second Strand Marker Master Mix

TruSeq Stranded mRNA Library Prep (96 Samples)

A quantity of two of each box is included for the 96 sample workflow.

Box 1
Quantity Reagent Description Storage
2 RPB RNA Purification Beads 2°C to 8°C
1 DTE CTE Dilution Tube Room temperature
1 DTA CTA Dilution Tube Room temperature
1 DTL CTL Dilution Tube Room temperature
Box2
Quantity Reagent Description Storage
2 BBB Bead Binding Buffer 2°C to 8°C
2 BWB Bead Washing Buffer 2°C to 8°C
2 ELB Elution Buffer 2°C to 8°C
2 FPF Fragment, Prime, Finish Mix -25°C to -15°C
Core Library Prep Box 1, Store at -25°C to -15°C
Quantity Reagent Description
1 ATL A-Tailing Mix
1 CTA A-Tailing Control
1 CTE End Repair Control
1 CTL Ligation Control
1 LIG Ligation Mix
1 RSB Resuspension Buffer
1 STL Stop Ligation Buffer
Core Library Prep Box 2, Store at -25°C to -15°C
Quantity Reagent Description
2 PMM PCR Master Mix
2 PPC PCR Primer Cocktail
2 FSA First Strand Synthesis Act D Mix
2 SMM Second Strand Marker Master Mix
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