This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.
After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
Recorded Webinar (January 2020)| Illumina Technical Support invites you to join us to discuss the end-to-end workflow for an amplicon sequencing project. This webinar is ideal for new users of Illumina Next Generation Sequencing. We will discuss amplicon sequencing, including the following topics: what defines an amplicon library, kits and protocols for amplicon libraries, and amplicon sequencing runs and parameters.
Recorded Webinar (March 2017) | Are you planning on running an Illumina amplicon library prep kit and wondering how to evaluate your data? This webinar is targeted to those who are new to amplicon sequencing and would like to learn more about the data output and assessment. We will cover the following subjects: overview of an Illumina amplicon workflow, expected kit specifications, Illumina options for amplicon analysis, output metrics and reports generated by the Illumina analysis platforms.
Recorded Webinar (January 2021) | FFPE is a widely used process to conserve tissues and generally contain partially degraded or limited genomic material. Specific considerations are required to sequence these challenging samples. This webinar is targeted towards both new and experienced users, with a basic knowledge of working with FFPE samples and will go over the following topics: a basic introduction to FFPE sample types, the nucleic acid extraction process, and library Preparation options.
Upcoming Webinar | Illumina invites you to join us to discuss evaluation of amplicon sequencing. This webinar is targeted for new and intermediate users of Illumina Next Generation Sequencing. In this webinar, we will review sequencing run performance for different Illumina amplicon sequencing workflows, including AmpliSeq and 16S, and analysis results. Our discussion will cover the following: optimal sequencing run set up, evaluating sequencing performance, supplementary analysis files, amplicon analysis options, key analysis metrics.
Jun 24, 2021 PST
Upcoming Webinar | Illumina Technical Support invites you to join us to discuss the end-to-end workflow for an amplicon sequencing project. This webinar is ideal for new users of Illumina Next Generation Sequencing. We will discuss amplicon sequencing, including the following topics: what defines an amplicon library, kits and protocols for amplicon libraries, and amplicon sequencing runs and parameters.
May 18, 2021 PST
Upcoming Webinar | Does my library look good to sequence? What’s that peak? This webinar will address how to use the Agilent BioAnalyzer to check library quality prior to sequencing, and troubleshoot sample preparation. This webinar is geared toward new and intermediate users, and will cover how to identify features of an ideal library trace, recognize potential issues, and prevent potential issues.
Apr 27, 2021 PST