NovaSeq 6000
Illumina recommends setting up a paired-end run with 151 cycles per read (2 × 151) and 8 cycles per index read.
TSO500 ctDNA Local App v1.0.1
Illumina DRAGEN Bio-IT Platform v3 only (DRAGEN On-Premise)
The license quota is based on gigabases. Use the following approximations to estimate the number of runs remaining on a DRAGEN TruSight Oncology 500 ctDNA Analysis Software license:
| NovaSeq 6000 Flow Cell | Data (gigabases) | Number of Libraries | Data per Library (gigabases) |
|---|---|---|---|
| S2 | 1000-1250 | 6 | 166-208 |
| S2 | 1000-1250 | 8 | 125-156 |
| S4 | 2400-3000 | 16 | 150-187 |
| S4 | 2400-3000 | 24 | 100-125 |
For optimal coverage, use the following guidelines for the number of libraries per sequencing run.
| NovaSeq 6000 Flow Cell | Flow Cell Loading | Minimum Number of Libraries | Maximum Number of Libraries |
|---|---|---|---|
| S2 | OBEM/Standard | 6 | 8 |
| S4 | OBEM/Standard | 16 | 16 |
| S4 | Xp | 6 libraries per lane | 6 libraries per lane |