NovaSeq 6000
Illumina recommends setting up a paired-end run with 151 cycles per read (2 × 151) and 8 cycles per index read.
TSO500 ctDNA Local App v1.0.1 (compatible with v1 sequencing consumables only)
TSO500 ctDNA Local App v1.1 (compatible with both v1 and v1.5 sequencing consumables)
Illumina DRAGEN Bio-IT Platform v3 only (DRAGEN On-Premise)
The license quota is based on gigabases. DRAGEN v3.6 subtracts a fixed 154 Gb per sample from the quota.
For optimal coverage, use the following guidelines for the number of libraries per sequencing run.
| NovaSeq 6000 Flow Cell | Flow Cell Loading | Minimum Number of Libraries | Maximum Number of Libraries |
|---|---|---|---|
| S2 | OBEM/Standard | 6 | 8 |
| S4 | OBEM/Standard | 16 | 16 |
| S4 | Xp | 6 libraries per lane | 6 libraries per lane |