TruSight Oncology 500 ctDNA Compatible Products

Compatible Sequencing Systems

NovaSeq 6000

Settings for Sequencing

Illumina recommends setting up a paired-end run with 151 cycles per read (2 × 151) and 8 cycles per index read.

Compatible Analysis Software

TSO500 ctDNA Local App v1.0.1

Compatible Analysis Hardware

Illumina DRAGEN Bio-IT Platform v3 only (DRAGEN On-Premise)

TSO 500 ctDNA/DRAGEN Compatibility Information

DRAGEN TruSight Oncology ctDNA Analysis Software License

The license quota is based on gigabases. Use the following approximations to estimate the number of runs remaining on a DRAGEN TruSight Oncology 500 ctDNA Analysis Software license:

NovaSeq 6000 Flow Cell Data (gigabases) Number of Libraries Data per Library (gigabases)
S2 1000-1250 6 166-208
S2 1000-1250 8 125-156
S4 2400-3000 16 150-187
S4 2400-3000 24 100-125
Number of Libraries/Compatibility

For optimal coverage, use the following guidelines for the number of libraries per sequencing run.

NovaSeq 6000 Flow Cell Flow Cell Loading Minimum Number of Libraries Maximum Number of Libraries
S2 OBEM/Standard 6 8
S4 OBEM/Standard 16 16
S4 Xp 6 libraries per lane 6 libraries per lane