HiSeq 2500 Primers

Catalog IDs: FC-121-1003, PE-121-1003

Sequencing Primers

Some dual-indexed libraries require sequencing primers in the TruSeq Dual Index Sequencing Primer Box. These primers replace primers provided in the reagent kits:

  • Read 1 Sequencing Primer—Load HP10 on the cBot 2 or cBot System instead of HP6.
  • Index 1 Read Sequencing Primer—Load HP12 instead of HP8.
  • Index 2 Read Sequencing Primer—For single-read flow cells, load HP9. For paired-end flow cells, load RMX from the TruSeq PE Cluster Kit v3.
  • Read 2 Sequencing Primer—Load HP11 instead of HP7.

For information on compatible libraries, see HiSeq 2500 Requirements & Compatibility. For clustering and sequencing instructions, see the cBot 2 System Guide or cBot System Guide and the HiSeq 2500 System Guide.

Contents and Storage

TruSeq Dual Index Sequencing Primer Box, Single-Read, Store at -25° to -15°C

Reagent Name

Storage

Description

HP9

-25° to -15°C

Index 2 (i5) Sequencing Primer Mix

HP10

-25° to -15°C

Read 1 Sequencing Primer Mix

HP12

-25° to -15°C

Index 1 (i7) Sequencing Primer Mix

TruSeq Dual Index Sequencing Primer Box, Paired-End, Store at -25° to -15°C

Reagent Name

Storage

Description

HP10

-25° to -15°C

Read 1 Sequencing Primer Mix

HP11

-25° to -15°C

Read 2 Sequencing Primer Mix

HP12

-25° to -15°C

Index 1 (i7) Sequencing Primer Mix