HiSeq 2500 High Output Kits

Catalog IDs: PE-401-4001, GD-401-4001, FC-401-4002, FC-401-4003, PE-401-3001, GD-401-3001, FC-401-3001, FC-401-3002

Kit Requirements

Performing a high output run requires one of the following kit combinations, depending on run mode. All kits are single-use.

  • HiSeq v4 mode—HiSeq Cluster Kit v4 and HiSeq SBS Kit v4
  • TruSeq v3 mode—TruSeq Cluster Kit v3 and TruSeq SBS Kit v3

The cluster kits are available in available in paired-end (PE) and single-read (SR) versions. Both versions contain clustering reagents for the cBot 2 or cBot System and indexing and paired-end reagents for the HiSeq 2500 System. The SBS kits include sequencing reagents to sequence one flow cell on the HiSeq 2500 System.

For other system requirements, dual indexing considerations, and compatible products, see HiSeq 2500 Requirements & Compatibility.

Contents and Storage

When you receive your kits, promptly store kit components at the indicated temperatures to ensure proper performance:

HiSeq Cluster Kit v4

HiSeq Cluster Kit v4 reagents are shipped in two boxes on dry ice. A flow cell, manifold, and accessories are also included.

Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Reagent Plate

PE kit—cBot PE reagent plate

SR kit—cBot SR reagent plate

PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Kit Component

Description

1

AMS

Fast Amplification Mix, High Output

1

FDR

Fast Denaturation Reagent, High Output (contains formamide)

1

FPM

Fast Amplification Premix, High Output

1

FRM

Fast Resynthesis Mix, High Output

1

FLM2

Fast Linearization Mix 2, High Output

1

HP11

Read 2 Primer Mix, High Output

1

HP12

Index i7 Primer Mix, High Output

SR Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Kit Component

Description

1

FDR

Fast Denaturation Reagent, High Output

1

HP12

Index 1 (i7) Primer Mix, High Output

1

HP9

Index 2 (i5) Primer Mix, High Output

Accessory Kit

Quantity

Accessory

Purpose

6

Flow cell gaskets

Replace flow cell gaskets between runs. Set contains 8-port gaskets (4) and 10-port gaskets (2).

8

Funnel bottle caps

Replace SBS reagent bottle caps before loading reagents.

HiSeq SBS Kit v4

HiSeq SBS Kit v4 reagents are shipped in two boxes. Reagents are sensitive to light. Store in a dark location away from light.

SBS Kit Box 1

Quantity

Reagent

Description

Storage Temperature

1

PW1

Wash Buffer

-25°C to 30°C

1

SB1

High Salt Buffer

2°C to 8°C

2

SB2

Incorporation Buffer

2°C to 8°C

1

SB3

Cleavage Buffer

2°C to 8°C

SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Storage Temperature

Description

1

IRM

-25°C to -15°C

Incorporation Reagent Master Mix

1

CRM

-25°C to -15°C

Cleavage Reagent Mix

1

USM

-25°C to -15°C

Universal Scan Mix

TruSeq Cluster Kit v3

TruSeq Cluster Kit v3 reagents are shipped in four boxes on dry ice.  A flow cell, manifold, and accessories are also included.

PE Cluster Kit Box 1, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HP3

Denaturation Solution

1

HT2

Wash Buffer

1

RMR

Resynthesis Mix

1

AT2

100% Formamide

1

APM2

Amplification Premix

1

AMX2

Amplification Mix 2

1

LMX2

Linearization Mix 2

1

BMX

Blocking Mix

1

HP7*

Read 2 Sequencing Primer Mix

* Instead of HP7, Nextera libraries require HP11 provided in the TruSeq Dual Index Sequencing Primer Box.

PE Cluster Kit Box 2, Store at -25°C to -15°C

Quantity

Kit Component

Description

1

cBot Reagent Plate

cBot PE reagent plate

1

HT1

Hybridization Buffer

SR Cluster Kit Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

cBot Reagent Plate

cBot SR reagent plate

1

HT1

Hybridization Buffer

Multiplex Sequencing Primer Box, Store at -25°C to -15°C

Quantity

Reagent

Description

1

HP3

Denaturation Solution

1

HT2

Wash Buffer

1

HP8*

Index Sequencing Primer

* Instead of HP8, Nextera libraries require HP12 provided in the TruSeq Dual Index Sequencing Primer Box.

Accessory Kit

Quantity

Component

Purpose

4

Flow cell gaskets

Replace the flow cell gaskets between runs.

8

Funnel bottle caps

Replace SBS reagent bottle caps before loading reagents.

1

ICB instrument tube

Divide ICB for Read 2.

TruSeq SBS Kit v3

TruSeq SBS Kit v3 reagents are shipped in two boxes on dry ice.

Reagents are labeled with a three-letter acronym plus a numeric designator that indicates the kit size. For example, if you are using CMR-50, the CMR was provided in a 50-cycle kit.

Box 1

Quantity

Reagent

Description

Storage Temperature

1

PW1

Wash Buffer

-25°C to 30°C

1

ICB

Incorporation Mix Buffer

2°C to 8°C

1

SB1

High Salt Buffer

2°C to 8°C

2

SB2

Incorporation Wash Buffer

2°C to 8°C

1

SB3

Cleavage Buffer

2°C to 8°C

Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

CMR

Cleavage Mix Reagent

1

SRE

Enhanced Scan Mix Reagent

1

EDP

Enhanced DNA Polymerase

4—200-cycle kit
1—50-cycle kit

LFN36

Long Read Nucleotide Mix