TruSight Cystic Fibrosis Contents & Storage

Contents and Storage

TruSight Cystic Fibrosis Library Prep, Box 1
Box 1A Pre-Amp Reagents
Component Quantity Storage
Cystic Fibrosis Oligo Pool 1 tube -25°C to -15°C
Hybridization Buffer 1 tube -25°C to -15°C
Extension-Ligation Mix 1 tube -25°C to -15°C
Index 2 Primers (A501-A508) 1 tube per primer -25°C to -15°C
Index 1 Primers (A701-A712)     1 tube per primer -25°C to -15°C
PCR Polymerase 1 tube -25°C to -15°C
PCR Master Mix 1 tube -25°C to -15°C
Box 1B Post-Amp Reagents
Component Quantity Storage
Library Normalization Diluent 1 tube -25°C to -15°C
Library Dilution Buffer 1 tube -25°C to -15°C
PhiX Internal Control 1 tube -25°C to -15°C
TruSight Cystic Fibrosis Library Prep, Box 2
Box 2 Pre-Amp Reagents
Component Quantity Storage
Filter Plate 4 plates 15°C to 30°C
Box 2 Post-Amp Reagents
Component Quantity Storage
Elution Buffer 1 tube 15°C to 30°C
Library Storage Buffer 1 tube 15°C to 30°C
TruSight Cystic Fibrosis Library Prep, Box 3
Box 3A Pre-Amp Reagents
Component Quantity Storage
Stringent Wash Buffer 1 bottle 2°C to 8°C
Universal Wash Buffer 1 tube 2°C to 8°C
Box 3B Post-Amp Reagents
Component Quantity Storage
PCR Clean-Up Beads
1 tube
2°C to 8°C
Library Normalization Wash 2 tubes 2°C to 8°C
Library Beads 1 tube 2°C to 8°C